NEW METHOD FOR PREPARATION OF PHOSPHOLIPID VESICLES (LIPOSOMES) - FRENCH PRESS
NEW METHOD FOR PREPARATION OF PHOSPHOLIPID VESICLES (LIPOSOMES) - FRENCH PRESS
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DOI：
10.1016/0014-5793(79)80281-1
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发表时间：
1979-01-01
期刊：
影响因子：
3.5
通讯作者：
LICHTENBERG, D
中科院分区：
文献类型：
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作者：
BARENHOLZT, Y;AMSELEM, S;LICHTENBERG, D
最近，人们对磷脂囊泡（脂质体）的兴趣越来越大，不仅作为模型膜，而且作为药物载体和作为将各种物质引入细胞或整个动物的工具[1 - 3]。目前已开发了至少5种不同的脂质体制备方法。超声辐照导致小的（~250 a直径）的形成。单层囊泡（WV）[4 - 61，当磷脂和胆汁盐的水性混合分散体通过SephadexG-50柱时，形成类似平均尺寸的囊泡[7]。从磷脂-去污剂分散体透析去污剂构成制备囊泡的另一种方法。这一过程导致形成平均粒径为1200~8 μ m的脂质体异质群体。特别适用于蛋白质包含在形成的大单层囊泡（LUV）[ %-IO]中的重构实验。当将脂质在有机溶剂中的混合物注射到加热的水溶液中时，形成甚至更大的囊泡[11 - 131;这些脂质体的大小实际上取决于溶剂、注射速率和水溶液的温度。非常大的囊泡（直径约1 μ m）当将磷脂的醚12或石油醚131溶液注入70 ℃的水溶液中时形成。类似大小的脂质体也可从含有SUV的磷脂酰丝氨酸（P）中获得，
Recently there has been a growing interest in phospholipid vesicles (liposomes) not only as model membranes but also as carriers of drugs and as a tool for the introduction of various substances into cells or in the whole animal [l-3]. At least 5 different methods have been developed for preparation of liposomes. Ultrasonic irradiation leads to the formation of small (-250 a diam.) unilamellar vesicles (WV)[4-61, Vesicles of a similar average size are formed when aqueous mixed dispersions of phospholipids and bile salts are passed through a Sephadex G-50 column [7].Dialysis of detergents from phospholipid-detergent dispersions constitute another method for the preparation of vesicles. This procedure, which leads to the formation of heterogeneous population of liposomes with av,-1200 8, diam. is especially suitable for reconstitution experiments in which proteins are included in the formed large unilamellar vesicles (LUV)[%-IO]. Even larger vesicles are formed when mixtures of lipids in organic solvents are injected into heated aqueous solutions [1 l-131; the size of these liposomes indeed depends on the solvent, rate of injection and temperature of the aqueous solution. Very large vesicles (-1 pm diam.) are formed when etheric 12 or petroletheric [131 solutions of phospholipids are injected into aqueous solutions at 70 C. Liposomes of similar sizes are also obtained from phosphatidylserine (P’s) containing SUV when they