RETROVIRUS RECOMBINATION DEPENDS ON THE LENGTH OF SEQUENCE IDENTITY AND IS NOT ERROR-PRONE
RETROVIRUS RECOMBINATION DEPENDS ON THE LENGTH OF SEQUENCE IDENTITY AND IS NOT ERROR-PRONE
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DOI：
10.1128/jvi.68.4.2409-2414.1994
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发表时间：
1994-04-01
影响因子：
5.4
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
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作者：
ZHANG, JY;TEMIN, HM
逆转录病毒由于其病毒体中存在两个相同的基因组RNA分子，因此以高速率重组。当非同源RNA存在于二聚体RNA分子中时，可以发生非同源重组，尽管其速率非常低，仅为基本同源重组速率的0.1%（J. Zhang和H. M. Temin，Science 259：234-238，1993）。我们发现，正如在天然存在的高致癌性逆转录病毒中发现的那样（J. Zhang和H. M. Temin，J. Virol. 67：1747-1751，1993），交叉通常发生在序列同一性的短区域。我们修改了先前研究的载体，以研究不同长度的短区域的序列同一性的影响，否则不相同的序列。我们发现，重组的效率取决于该序列同一性的长度。然而，即使当存在广泛的序列同一性时，此类分子中的最高速率仍然低于基本上同源的分子之间的重组。重组体的连接序列表明，逆转录病毒重组不是一个容易出错的过程，正如对人免疫缺陷病毒逆转录酶使用无细胞系统所报道的那样（J. A. Peliska和S. J. Benkovic，Science 258：1112-1118，1992）。
Retroviruses, as a result of the presence of two identical genomic RNA molecules in their virions, recombine at a high rate. When nonhomologous RNA is present in the dimer RNA molecules, nonhomologous recombination can occur, although the rate is very low, only 0.1% of the rate of essentially homologous recombination (J. Zhang and H. M. Temin, Science 259:234-238, 1993). We found, as is found in naturally occurring highly oncogenic retroviruses (J. Zhang and H. M. Temin, J. Virol. 67:1747-1751, 1993), that the crossovers usually occur at a short region of sequence identity. We modified the previously studied vectors to study the effect of different lengths of short regions of sequence identity in the midst of otherwise nonidentical sequences. We found that the efficiency of recombination depends on the length of this sequence identity. However, the highest rate in such molecules remained lower than for recombination between essentially homologous molecules, even when there was extensive sequence identity. Junction sequences of the recombinants indicated that retrovirus recombination is not an error-prone process as was reported for human immunodeficiency virus reverse transcriptase by using a cell-free system (J. A. Peliska and S. J. Benkovic, Science 258:1112-1118, 1992).