Measurement of acetylcholine released from rabbit detrusor smooth muscle using HPLC with electro-chemical detection coupled with microdialysis procedure.
Measurement of acetylcholine released from rabbit detrusor smooth muscle using HPLC with electro-chemical detection coupled with microdialysis procedure.
复制标题
使用 HPLC 结合电化学检测和微透析程序测量兔逼尿肌平滑肌释放的乙酰胆碱。
DOI：
10.1016/s0024-3205(98)00228-8
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发表时间：
1998
期刊：
影响因子：
6.1
通讯作者：
S. Ueda
中科院分区：
文献类型：
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作者：
A. Inadome;M. Yoshida;W. Takahashi;Y. Wada;K. Kitani;H. Kikukawa;M. Yono;H. Seshita;S. Ueda
用微透析法测定了电场刺激(EFS)诱导的兔逼尿肌的乙酰胆碱(ACh)释放量。透析探头经逼尿肌条插入，用含有硫酸毒扁豆碱的林格液以2min L/μ的速度持续灌流。将试纸悬浮在装满改良Krebs-Henseleit溶液的器官浴缸中，然后输送EFS。记录并监测每个肌肉准备中的等长力。每10分钟收集一次透析液。采用高效液相色谱-电化学检测法测定ACh。EFS引起的肌条收缩和ACh释放呈频率和时间依赖性增加。收缩和频率依赖性ACh释放的频率反应曲线有一定差异。在收缩反应中，最大收缩出现在较低频率，而ACh释放在较高频率达到最大。在EFS诱导的收缩和ACh释放之间存在显著的，但不是简单的相关性。结果提示，这种新方法可用于研究兔逼尿肌ACh的释放，除ACh外，其他神经递质可能参与EFS引起的收缩。
We measured the amount of acetylcholine (ACh) released from rabbit detrusor smooth muscles induced by electrical field stimulation (EFS) using microdialysis procedure. The dialysis probe was inserted through the detrusor muscle strip and was continuously perfused with a Ringer solution containing physostigmine sulfate, at a rate of 2 μl/min. The strip was suspended in an organ bath filled with the modified Krebs-Henseleit solution and then EFS was delivered. The isometric force was recorded and monitored in each muscle preparation. The dialysates were collected every 10 min. Ach was determined by a high performance liquid chromatography with electro-chemical detection. The contraction of the muscle strip and ACh release induced by EFS were increased in a frequency and duration dependent manner. There were some differences between frequency response curves of contraction and frequency dependent ACh release. In the contractile response, the maximum contractions were observed at lower frequencies, while ACh releases reached the maximum at higher frequencies. There was a significant, but not simple correlation between EFS-induced contraction and ACh release. The results suggest that this new method is useful to investigate the ACh release from rabbit detrusor smooth muscles, and that other neurotransmitters than ACh possibly contribute to EFS-induced contraction.
DOI：
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