Genetic control of developmental changes induced by disruption of Arabidopsis histone deacetylase 1 (AtHD1) expression.
Genetic control of developmental changes induced by disruption of Arabidopsis histone deacetylase 1 (AtHD1) expression.
复制标题
拟南芥组蛋白脱乙酰酶 1 (AtHD1) 表达破坏诱导的发育变化的遗传控制。
DOI：
10.1093/genetics/165.1.399
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发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Chen,ZJeffrey
中科院分区：
文献类型：
--
作者：
Tian,Lu;Wang,Jianlin;Fong,MPaulus;Chen,Meng;Cao,Hongbin;Gelvin,StantonB;Chen,ZJeffrey
关于遗传和表观遗传控制在植物发育的空间和时间调控中的作用，人们知之甚少。高表达反义拟南芥HD1(AtHD1)编码一个主要的组蛋白脱乙酰基酶，对植物的生长和发育具有多效性。目前尚不清楚这些发育异常是由缺陷型ATHD1或相关同源物引起的，并且在自交后代中是可遗传的。我们分离到一个稳定的反义AtHD1(Cash)转基因品系和一个位于AtHD1外显子2的T-DNA插入系，得到一个空等位基因(athd1-t1)。在纯合(athd1-t1/athd1-t1)植株的后代中，组蛋白乙酰化水平和类似的发育异常均可在反义AtHD1存在时遗传。此外，当athd1-t1/athd1-t1植株与野生型植株杂交时，F1代的多效性发育异常立即恢复，这与AtHD1表达和组蛋白H4 Lys12乙酰化减少有关。与DNA和组蛋白甲基化稳定密码子的情况不同，组蛋白脱乙酰酶缺陷引起的发育变化是立即可逆的，可能是通过恢复正常控制基因调控和发育所需的可逆组蛋白乙酰化密码子来实现的。
Little is known about the role of genetic and epigenetic control in the spatial and temporal regulation of plant development. Overexpressing antisenseArabidopsis thaliana HD1(AtHD1) encoding a putative major histone deacetylase induces pleiotropic effects on plant growth and development. It is unclear whether the developmental abnormalities are caused by a defectiveAtHD1or related homologs and are heritable in selfing progeny. We isolated a stable antisenseAtHD1(CASH) transgenic line and a T-DNA insertion line in exon 2 ofAtHD1, resulting in a null allele (athd1-t1). Bothathd1-t1and CASH lines display increased levels of histone acetylation and similar developmental abnormalities, which are heritable in the presence of antisenseAtHD1or in the progeny of homozygous (athd1-t1/athd1-t1) plants. Furthermore, when theathd1-t1/athd1-t1plants are crossed to wild-type plants, the pleiotropic developmental abnormalities are immediately restored in the F1hybrids, which correlates withAtHD1expression and reduction of histone H4 Lys12 acetylation. Unlike the situation with the stable code of DNA and histone methylation, developmental changes induced by histone deacetylase defects are immediately reversible, probably through the restoration of a reversible histone acetylation code needed for the normal control of gene regulation and development.