Expression patterns and association analysis of the porcine DHX58 gene
Expression patterns and association analysis of the porcine DHX58 gene
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猪DHX58基因的表达模式及关联分析
DOI：
10.1111/j.1365-2052.2010.02027.x
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发表时间：
2010-10-01
期刊：
影响因子：
2.4
通讯作者：
Zhao, S. H.
中科院分区：
文献类型：
--
作者：
Li, X. Y.;Han, C. M.;Zhao, S. H.
P>；RIG-1信号转导系统负责检测细胞质病毒RNA分子。DXH(Asp-Glu-X-His)盒多肽58(由DHX58编码)是RIG-1信号通路的负调控因子。在人类中，DHX58基因可以上调，并在病毒感染过程中抑制RIG-1信号通路。本研究对猪DHX58基因的表达模式进行了研究。根据我们的结果，猪DHX58基因不仅被Poly I：C刺激上调，而且还被脂多糖(LPS)刺激上调。在第9个内含子上检测到一个多态(g.4919G>；C)，该多态与1日龄猪的红细胞分布宽度和17日龄猪的白细胞数、淋巴细胞绝对数和血小板分布宽度等血液参数显著相关(P<；0.05)。此外，GG基因携带者的平均白细胞计数显著高于CC或GC基因携带者(P<；0.05)。我们的研究表明，DHX58是一个与猪的免疫反应相关的重要基因。
P>RIG-1 signalling is responsible for the detection of cytoplasmic viral RNA molecules. DEXH (Asp-Glu-X-His) box polypeptide 58 (encoded by DHX58) is a negative regulator of the RIG-1 signalling pathway. In human, the DHX58 gene can be upregulated and can inhibit the RIG-1 signalling pathway during viral infection. In this study, porcine DHX58 gene expression patterns were studied. According to our results, the porcine DHX58 gene was upregulated not only by the stimulation of Poly I:C but also by the stimulation of 1ipopolysaccharides (LPS). One polymorphism (g.4919G > C), detected in the ninth intron, was significantly associated with some blood parameters including the red cell distribution width of 1-day-old pigs and white blood cell counts, lymphocyte absolute counts, and platelet distribution width of 17-day-old pigs (P < 0.05). Moreover, the individuals with the genotype GG have a significantly higher mean white blood cell count than individuals with genotype CC or GC (P < 0.05). Our study indicates that DHX58 is an important gene that is associated with the immune response in swine.