Cell-free synthesis and glycosylation of the major human-red-cell sialoglycoprotein, glycophorin A.
Cell-free synthesis and glycosylation of the major human-red-cell sialoglycoprotein, glycophorin A.
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主要人红细胞唾液酸糖蛋白、糖蛋白 A 的无细胞合成和糖基化。
DOI：
10.1111/j.1432-1033.1981.tb05159.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Gahmberg,CG
中科院分区：
文献类型：
--
作者：
Jokinen,M;Ulmanen,I;Andersson,LC;Kääriäinen,L;Gahmberg,CG
我们从K562细胞中分离出编码糖蛋白A的mRNA片段，并在兔网织红细胞无细胞系统中研究了该唾液糖蛋白的合成。在没有膜的情况下，合成了糖蛋白a的前体形式。这是用特异性抗（糖蛋白A）血清鉴定的。糖蛋白A无碳水化合物前体wss 19500的表观分子量。这比糖蛋白A载脂蛋白的分子量大约高出5000倍。在狗胰腺膜存在的情况下，合成的糖蛋白A前体没有糖基化，也可能是糖基化。低聚糖链仍然不完整，体外合成的糖蛋白与完整细胞中获得的糖蛋白A的糖基化前体相对应。
The human erythroid cell line, K562, synthesizes the major red cell sialoglycoprotein, glycophorin A. We have isolated an mRNA fraction which codes for glycophorin A from K562 cells and studied the synthesis of the sialoglycoprotein in a rabbit reticulocyte cell‐free system. In the absence of membranes a precursor form of glycophorin A was synthesized. This was identified using specific anti‐(glycophorin A) serum. The apparent molecular weight of the carbohydrate‐free precursor of glycophorin A wss 19500. This exceeds the molecular weight of the glycophorin A apoprotein by approximately 5000. In the presence of membranes from dog pancreas, the synthesized glycophorin A precursor wasN‐glycosylated and probably alsoO‐glycosylated. The oligosaccharide chains remained incompleted and the glycoprotein synthesizedin vitrocorresponded to the glycosylated precursor of glycophorin A obtained in intact cells.