Effective and Reduced-Cost Modified Selective Medium for Isolation of Clostridium difficile
Effective and Reduced-Cost Modified Selective Medium for Isolation of Clostridium difficile
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DOI：
10.1128/jcm.01591-08
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发表时间：
2009-02-01
影响因子：
9.4
通讯作者：
Donskey, Curtis J.
中科院分区：
文献类型：
--
作者：
Nerandzic, Michelle M.;Donskey, Curtis J.
无论是流行病学研究还是诊断测试，都需要有效、经济且易于获得的选择性培养基来培养艰难梭菌。我们开发了一种环丝氨酸-头孢西丁-果糖琼脂 (CCFA) 的低成本替代品，它是一种有效但昂贵的艰难梭菌选择性培养基。这种改良培养基称为艰难梭菌布鲁氏菌琼脂 (CDBA)，包含富集的布鲁氏菌碱作为蛋白胨 1 号的替代品。 2、牛磺胆酸钠浓度由0.1%降低至0.05%。为了比较 CDBA 和 CCFA 的敏感性和选择性，使用患有艰难梭菌相关疾病的患者的粪便样本进行艰难梭菌培养。 CDBA 对艰难梭菌的恢复与 CCFA 一样敏感，粪便微生物群突破生长的频率相似（分别为 25% 和 31%）。改良培养基的液体制剂，称为艰难梭菌布鲁氏菌肉汤（CDBB），可刺激艰难梭菌孢子快速萌发和生长，其速度与环丝氨酸-头孢西丁-果糖肉汤相当。我们的结果表明，CDBA 和 CDBB 是从粪便样本中回收艰难梭菌的敏感、选择性和成本降低的介质。
Both for epidemiologic studies and for diagnostic testing, there is a need for effective, economical, and readily available selective media for the culture of Clostridium difficile. We have developed a reduced-cost substitute for cycloserine-cefoxitin-fructose agar (CCFA), which is an effective but expensive selective medium for C. difficile. The modified medium, called C. difficile brucella agar (CDBA), includes an enriched brucella base as a substitute for proteose peptone no. 2, and the concentration of sodium taurocholate has been reduced from 0.1% to 0.05%. To compare the sensitivities and selectivities of CDBA and CCFA, cultures for C. difficile were performed using stool samples from patients with C. difficile-associated disease. CDBA was as sensitive as CCFA for the recovery of C. difficile, with a similar frequency of breakthrough growth of stool microflora (25% versus 31%, respectively). A liquid formulation of the modified medium, termed C. difficile brucella broth (CDBB), stimulated rapid germination and outgrowth of C. difficile spores, at a rate comparable to that in cycloserine-cefoxitin-fructose broth. Our results suggest that CDBA and CDBB are sensitive, selective, and reduced-cost media for the recovery of C. difficile from stool samples.