GLUCOSE-REGULATED PROTEIN (GRP94 AND GRP78) GENES SHARE COMMON REGULATORY DOMAINS AND ARE COORDINATELY REGULATED BY COMMON TRANS-ACTING FACTORS
GLUCOSE-REGULATED PROTEIN (GRP94 AND GRP78) GENES SHARE COMMON REGULATORY DOMAINS AND ARE COORDINATELY REGULATED BY COMMON TRANS-ACTING FACTORS
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DOI：
10.1128/mcb.9.5.2153
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发表时间：
1989-05-01
影响因子：
5.3
通讯作者：
LEE, AS
中科院分区：
文献类型：
--
作者：
CHANG, SC;ERWIN, AE;LEE, AS
我们分离出了编码 94,000 道尔顿葡萄糖调节蛋白 (GRP94) 的人类基因的启动子。通过缺失分析鉴定了对其表达重要的5''侧翼区域。对源自人、大鼠和鸡细胞的 GRP78 和 GRP94 基因启动子的比较揭示了两个基因的推定调控区域内的 28 个碱基对的共同结构域。该结构域已被证明与 GRP78 基因启动子中的蛋白质因子相互作用。由于 GRP94 和 GRP78 的基因在各种应激条件下以相似的动力学进行转录调控，因此我们有兴趣检查它们协调表达的可能机制。通过体外和体内竞争实验，我们发现与GRP94基因启动子相互作用的蛋白因子也对GRP78基因启动子的保守结构域具有亲和力。这些发现表明，GRP94 和 GRP78 的基因通过共同的反式作用因子进行协调调节，这些反式作用因子识别葡萄糖相关蛋白基因启动子的共同调节域。
We isolated the promoter of the human gene encoding the 94,000-dalton glucose-regulated protein (GRP94). The 5''-flanking region important for its expression was identified by deletion analysis. Comparison of the promoters of the genes for GRP78 and GRP94 derived from human, rat, and chicken cells revealed a common domain of 28 base pairs within the putative regulatory regions of both genes. This domain has been shown to interact with protein factors in the promoter of the gene for GRP78. Since the genes for GRP94 and GRP78 are transcriptionally regulated with similar kinetics under a variety of stress conditions, we are interested in examining the possible mechanisms for their coordinated expression. Through in vitro and in vivo competition assays, we found that the protein factors which interact with the promoter of the gene for GRP94 also have affinity for the conserved domain of the promoter of the gene for GRP78. These findings suggest that the genes for GRP94 and GRP78 are coordinately regulated through common trans-acting factors which recognize a common regulatory domain of glucose-related protein gene promoters.