Apparent lack of tetrodotoxin biosynthesis in captured Taricha torosa and Taricha granulosa.
Apparent lack of tetrodotoxin biosynthesis in captured Taricha torosa and Taricha granulosa.
复制标题
捕获的 Taricha torosa 和 Taricha capsulosa 明显缺乏河豚毒素生物合成。
DOI：
10.1248/cpb.31.3625
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发表时间：
1983
影响因子：
1.7
通讯作者：
Masaru Kobayashi
中科院分区：
文献类型：
--
作者：
Y. Shimizu;Masaru Kobayashi
为了探究强效神经毒素河豚毒素的来源，利用[2-14C]-乙酸盐、[胍基-14C]-精氨酸、[脲基-14C]-瓜氨酸和[14C(U)]-葡萄糖对蝾螈Taricha torosa和Taricha capsulosa进行了饲养实验。通过注射、口服或将蝾螈浸泡在含有放射性前体的水浴中来施用前体。尽管胆固醇、胺、氨基酸和大分子化合物等常见代谢物被显着标记，但这些喂养实验均未导致河豚毒素中掺入放射性。发现用于实验的细粒木耳和托罗萨木耳的毒性比之前报道的要高。它们在圈养期间保留了高水平的毒性，但人们发现它们不断释放少量毒素（一年内总毒素的2.5%）。当受到电击时，蝾螈会释放出大量的毒素。
In order to explore the origin of the potent neurotoxin tetrodotoxin, feeding experiments were carried out using [2-14C]-acetate, [guanido-14C]-arginine, [ureido-14C]-citrulline and [14C (U)]-glucose on the newts Taricha torosa and Taricha granulosa. The precursors were administered by injection, by oral feeding, or by soaking the newts in a bath of water containing the radioactive precursors. None of these feeding experiments resulted in the incorporation of radioactivity into tetrodotoxin, though common metabolites such as cholesterol, amines, amino acids, and macromolecular compounds were significantly labeled. The toxicities of T. granulosa and T. torosa used for the experiment were found to be higher than those previously reported. They retained a high level of toxicity during their captivity, yet they were found to continuously release small amounts of toxins (2.5% of the total toxins in one year). When subjected to an electric shock, the newts released significant amounts of toxin.