Protein-repellent and antibacterial functions of a calcium phosphate rechargeable nanocomposite
Protein-repellent and antibacterial functions of a calcium phosphate rechargeable nanocomposite
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磷酸钙可充电纳米复合材料的蛋白质排斥和抗菌功能
DOI：
10.1016/j.jdent.2016.06.003
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发表时间：
2016-09-01
影响因子：
4.4
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
--
作者：
Xie, Xianju;Wang, Lin;Xu, Hockin H. K.
目的：我们最近开发了一种新的可充电复合材料，该复合材料含有具有长期钙(Ca)和磷(P)离子释放的无定形磷酸钙(NACP)纳米颗粒，但该复合材料并不抗菌。本研究的目的是：(1)将甲基丙烯酸二甲氨基十六酯(DMAHDM)和2-甲基丙烯酰氧乙基磷酰胆碱(MPC)复合到可充电的NACP复合材料中，研究复合材料的力学性能、蛋白质吸附和生物膜响应以及生物膜介质的pH值。方法：将MPC、DMAHDM和NACP混合到乙氧基双酚A二甲基丙烯酸酯(EBPADMA)和均苯四甲酸甘油二甲酯(PMGDM)树脂中。用微量比辛酸方法测定蛋白质的吸附。利用唾液微宇宙生物膜模型在复合材料上生长生物膜。测定菌落形成单位(CFU)、活/死试验、代谢活性和生物膜培养基pH。实验采用n=6。结果：含3%MPC的复合材料对蛋白质的吸附比市售复合材料低一个数量级(p<；0.05)。对照复合体完全被活细菌覆盖。3%MPC+3%DMAHDM的活菌数最少(p<；0.05)。含有3%MPC+3%DMAHDM的复合材料抑制了生物膜的生长和活性，与商业对照复合材料(p<；0.05)相比，生物膜CFU减少了3log，而弯曲强度与商业复合材料(p>；0.1)相近。含3%MPC+3%DMAHDM的复合体在生物膜培养液中的pH值维持在6.5以上，而商品化的复合体在生物膜培养液中的致龋性pH为4.2。结论：新型蛋白质驱避剂和抗菌NACP充电复合材料显著抑制了生物膜的生长，产生的pH值远高于商品复合体。临床意义：这种新型的生物活性纳米复合材料有望保护牙齿结构免受生物膜酸和龋病的侵袭。使用NACP、MPC和DMAHDM的方法也适用于其他牙科材料，以减少菌斑堆积和继发性龋病。(C)2016爱思唯尔有限公司。保留所有权利。
Objectives: We recently developed a new rechargeable composite with nanoparticles of amorphous calcium phosphate (NACP) having long-term calcium (Ca) and phosphate (P) ion release; however, this composite was not antibacterial. The objectives of this study were to: (1) incorporate dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacryloyloxyethyl phosphorylcholine (MPC) into rechargeable NACP composite, and (2) investigate mechanical properties, protein adsorption and biofilm response of composite, and the pH of biofilm medium.Methods: MPC, DMAHDM and NACP were mixed into a resin of ethoxylated bisphenol A dimethacrylate (EBPADMA) and pyromellitic glycerol dimethacrylate (PMGDM). Protein adsorption was measured using a micro bicinchoninic acid method. A human saliva microcosm biofilm model was used to grow biofilms on composites. Colony-forming units (CFU), live/dead assay, metabolic activity, and biofilm culture medium pH were determined. The tests used n = 6.Results: The composite with 3% MPC had protein adsorption an order of magnitude less than that of a commercial composite (p < 0.05). Control composites were fully covered by live bacteria. Live bacteria were reduced via MPC; 3% MPC + 3% DMAHDM had the least live bacteria (p < 0.05). The composite with 3% MPC + 3% DMAHDM inhibited biofilm growth and viability, reducing biofilm CFU by 3 log compared to commercial control composite (p < 0.05), while having a flexural strength similar to that of the commercial composite (p > 0.1). The composite containing 3% MPC + 3% DMAHDM with biofilm culture maintained a pH above 6.5, while the commercial composite had a cariogenic pH of 4.2 in biofilm culture medium.Conclusions: The new protein-repellent and antibacterial NACP rechargeable composite substantially reduced biofilm growth, yielding a much higher pH than a commercial composite.Clinical significance: This novel bioactive nanocomposite is promising to protect tooth structures from biofilm acids and caries. The method of using NACP, MPC and DMAHDM may be applicable to other dental materials to reduce plaque buildup and secondary caries. (C) 2016 Elsevier Ltd. All rights reserved.