Antiangiogenic effect of a Chinese sweet leaf tea extract in experimental corneal neovascularization
Antiangiogenic effect of a Chinese sweet leaf tea extract in experimental corneal neovascularization
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DOI：
10.1080/13880200601026333
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发表时间：
2007-01-01
影响因子：
3.8
通讯作者：
Woltering, Eugene A.
中科院分区：
文献类型：
--
作者：
Oner, F. Hakan;Bezerra, Yanno;Woltering, Eugene A.
本研究旨在评估局部施用悬钩子（蔷薇科）（RUS）（一种中国甜叶茶提取物）在预防实验诱导的角膜新生血管形成方面的效果。通过硝酸银烧灼技术在 40 只大鼠眼睛（每只动物 1 只）中诱导角膜新生血管形成。三种浓度（0.1%、1% 和 10% w/v）的 RUS 生理盐水局部给药，每天两次，持续 7 天。使用照片测量新生血管对角膜表面的覆盖率，作为角膜总面积的百分比。对每个角膜进行组织病理学评分。治疗组和安慰剂组的平均烧伤刺激评分差异无统计学意义（p = 0.714）。 10% RUS 溶液导致烧灼反应中新生血管的百分比显着下降 (p < 0.01)； 0.1% 和 1% 浓度与对照没有显着差异 (p = 0.173)。 10%浓度组的新生血管明显少于对照组（p < 0.01）。结果发现，与对照眼相比，局部应用 10% RUS 溶液导致硝酸银烧灼的新生血管反应显着降低。
This study was undertaken to evaluate the effect of topical administration of Rubus suavissimus S. Lee (Rosaceae) (RUS), an extract of Chinese sweet leaf tea, in the prevention of experimentally induced corneal neovascularization. Corneal neovascularization was induced in 40 rat eyes (1 per animal) by a silver nitrate cauterization technique. Three concentrations (0.1%, 1%, and 10% w/v) of RUS in saline were administered topically twice a day for 7 days. The coverage of the corneal surface by neovascular vessels was measured, using photographs, as a percentage of total cornea area. A histopathologic score was assigned to each cornea. The difference in mean burn stimulus scores of treatment and placebo groups was not statistically significant (p = 0.714). The 10% RUS solution caused a significant decrease in the percentage of neovascularization in response to cauterization (p < 0.01); the 0.1% and 1% concentrations were not significantly different from controls (p = 0.173). The 10% concentration group had significantly less neovascularization than the control group (p < 0.01). It was found that topical application of 10% RUS solution caused a significant decrease in neovascular response to silver nitrate cauterization compared with control eyes.