Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality
Number of biopsied trophectoderm cells is likely to affect the implantation potential of blastocysts with poor trophectoderm quality
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活检滋养外胚层细胞的数量可能会影响滋养外胚层质量差的囊胚的植入潜力
DOI：
10.1016/j.fertnstert.2016.01.011
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发表时间：
2016-05-01
影响因子：
6.7
通讯作者：
Lin, Ge
中科院分区：
文献类型：
--
作者：
Zhang, Shuoping;Luo, Keli;Lin, Ge
目的：评估胚胎发育潜力是否受到植入前遗传学诊断 (PGD) 周期中活检的滋养外胚层 (TE) 细胞数量的影响。设计：回顾性研究。地点：大学附属中心。患者：接受囊胚活检和荧光原位杂交分析的 PGD 周期的女性。干预措施：不适用。主要结果指标：活检 TE囊胚细胞数、存活率和着床率。结果：活检的 TE 细胞数受到不同胚胎学家 TE 质量和经验的影响。当活检 1 到 5 个细胞时，诊断效率会增加（86.7%、91.7%%、96.0%、96.8% 至 98.7%），当活检超过 6 个细胞时，诊断效率最大化。为了比较临床效率，根据活检的 TE 细胞数将囊胚分为四组：1-5、6-10、11-15 和 16-41。对于TE评分为A级的囊胚，四组间的存活率和着床率均无显着差异。对于TE评分为B级和C级的囊胚，四组之间的存活率没有显着差异，但随着活检TE细胞数量的增加，着床率出现显着下降的趋势。结论：TE形态评分较差的囊胚的着床潜力受到活检TE细胞数量的负面影响。 (C) 2016 年，美国生殖医学会。）
Objective: To evaluate whether the developmental potential of the blastocyst is affected by the number of trophectoderm (TE) cells biopsied in preimplantation genetic diagnosis (PGD) cycles.Design: Retrospective study.Setting: University-affiliated center.Patient(s): Women underwent PGD cycles of blastocyst biopsy and fluorescence in situ hybridization analysis.Intervention(s): Not applicable.Main Outcome Measure(s): Biopsied TE cell number of blastocysts, survival, and implantation rates.Result(s): The biopsied TE cell number was affected by the TE quality and experience of different embryologists. The diagnostic efficiency increased when from one to five cells were biopsied (86.7%, 91.7%%, 96.0%, 96.8%, to 98.7%) and was maximized when more than six cells were biopsied. To compare the clinical efficiencies, blastocysts were divided into four groups according to biopsied TE cell number: 1-5, 6-10, 11-15, and 16-41. For the blastocysts with grade A TE score, no significant difference was observed in the survival and implantation rates among the four groups. For the blastocysts with grades B and C TE scores, the survival rates showed no significant differences among the four groups, but a significant decreasing trend in implantation rates was observed with increasing biopsied TE cell number.Conclusion(s): The implantation potential is negatively affected by the biopsied TE cell number in blastocysts with poor TE morphological score. (C) 2016 by American Society for Reproductive Medicine.)