Tissue sample stability: thawing effect on multi-organ samples
Tissue sample stability: thawing effect on multi-organ samples
复制标题
组织样品稳定性：对多器官样品的解冻效果
作者：
Frida Torell;K. Bennett;S. Cereghini;S. Rännar;K. Lundstedt;T. Moritz;C. Haumaître;J. Trygg;T. Lundstedt
正确处理样品在代谢组学研究中至关重要。样本处理不当和储存时间过长会对代谢物水平产生不良影响。本研究的目的是确定解冻对不同器官样本的影响。在瑞典Umegalian大学的瑞典代谢组学中心，使用气相色谱飞行时间质谱法，以非靶向代谢组学方法分析了来自肠道、肾脏、肝脏、肌肉和胰腺的器官样本中的许多内源性代谢物。采用主成分分析和正交投影潜在结构判别分析对数据进行多元分析。结果表明，解冻引起的各器官代谢变化基本一致。正如预期，解冻后总体代谢物水平显著增加，这是由蛋白质和细胞降解增加引起的。胆固醇是所有器官组解冻样本中发现降低的8种代谢物之一。结果还表明，与其他器官样品相比，肌肉对氧化的敏感性较低。
Correct handling of samples is essential in metabolomic studies. Improper handling and prolonged storage of samples has unwanted effects on the metabolite levels. The aim of this study was to identify the effects that thawing has on different organ samples. Organ samples from gut, kidney, liver, muscle and pancreas were analyzed for a number of endogenous metabolites in an untargeted metabolomics approach, using gas chromatography time of flight mass spectrometry at the Swedish Metabolomics Centre, Umeå University, Sweden. Multivariate data analysis was performed by means of principal component analysis and orthogonal projection to latent structures discriminant analysis. The results showed that the metabolic changes caused by thawing were almost identical for all organs. As expected, there was a marked increase in overall metabolite levels after thawing, caused by increased protein and cell degradation. Cholesterol was one of the eight metabolites found to be decreased in the thawed samples in all organ groups. The results also indicated that the muscles are less susceptible to oxidation compared to the rest of the organ samples.