GmBTB/POZ, a novel BTB/POZ domain-containing nuclear protein, positively regulates the response of soybean to Phytophthora sojae infection
GmBTB/POZ, a novel BTB/POZ domain-containing nuclear protein, positively regulates the response of soybean to Phytophthora sojae infection
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GmBTB/POZ是一种新型含BTB/POZ结构域的核蛋白，正向调节大豆对大豆疫霉感染的反应
DOI：
10.1111/mpp.12741
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发表时间：
2019
影响因子：
4.9
通讯作者：
Zhang Shuzhen
中科院分区：
文献类型：
--
作者：
Zhang Chuanzhong;Gao Hong;Li Rongpeng;Han Dan;Wang Le;Wu Junjiang;Xu Pengfei;Zhang Shuzhen
大豆疫霉（Phytophthora sojae）是大豆[Glycine max（L.）梅尔。]它导致全世界大豆植株的茎和根腐烂。然而，植物对大豆疫霉防御反应的发病机理和分子机制尚不清楚。在此，我们记录了一种新的BTB/POZ蛋白，GmBTB/POZ，它包含在某些动物转录调节因子中发现的BTB/POZ结构域，在宿主大豆植物中响应大豆疫霉的潜在机制和功能。它位于细胞核中，并由大豆疫霉转录上调。GmBTB/POZ在大豆中的过量表达导致了对大豆疫霉的抗性增强。超氧化物歧化酶（SOD）和过氧化物酶（POD）抗氧化剂的活性和表达水平在GmBTB/POZ-过表达（GmBTB/POZ-OE）转基因大豆植株中显著高于用无菌水处理或感染大豆疫霉的野生型（WT）植株。防御相关基因的转录水平在过表达植物中也高于感染的WT。此外，GmBTB/POZ ‐ OE转基因大豆中水杨酸（SA）水平和SA生物合成相关基因的转录水平显著高于WT，但GmBTB/POZ ‐ OE和WT大豆品系之间茉莉酸（JA）水平和JA生物合成相关基因的表达几乎没有差异。此外，外源SA的施用诱导了野生型和转GmBTB/POZ-OE基因大豆植株中GmBTB/POZ的表达，并抑制了大豆疫霉生物量的增加。这些结果表明，GmBTB/POZ在大豆抗疫和防御反应中起着积极的作用，其作用可能依赖于SA。
Phytophthora sojaeis a destructive pathogen of soybean [Glycine max(L.) Merr.] which causes stem and root rot on soybean plants worldwide. However, the pathogenesis and molecular mechanism of plant defence responses againstP. sojaeare largely unclear. Herein, we document the underlying mechanisms and function of a novel BTB/POZ protein, GmBTB/POZ, which contains a BTB/POZ domain found in certain animal transcriptional regulators, in host soybean plants in response toP. sojae. It is located in the cell nucleus and is transcriptionally up‐regulated byP. sojae. Overexpression ofGmBTB/POZin soybean resulted in enhanced resistance toP. sojae. The activities and expression levels of enzymatic superoxide dismutase (SOD) and peroxidase (POD) antioxidants were significantly higher inGmBTB/POZ‐overexpressing (GmBTB/POZ‐OE) transgenic soybean plants than in wild‐type (WT) plants treated with sterile water or infected withP. sojae. The transcript levels of defence‐associated genes were also higher in overexpressing plants than in WT on infection. Moreover, salicylic acid (SA) levels and the transcript levels of SA biosynthesis‐related genes were markedly higher inGmBTB/POZ‐OE transgenic soybean than in WT, but there were almost no differences in jasmonic acid (JA) levels or JA biosynthesis‐related gene expression betweenGmBTB/POZ‐OE and WT soybean lines. Furthermore, exogenous SA application induced the expression ofGmBTB/POZand inhibited the increase inP. sojaebiomass in both WT andGmBTB/POZ‐OE transgenic soybean plants. Taken together, these results suggest that GmBTB/POZ plays a positive role inP. sojaeresistance and the defence response in soybean via a process that might be dependent on SA.