Towards easier, faster, super-resolved microscopy
Towards easier, faster, super-resolved microscopy
复制标题
迈向更简单、更快、超分辨率显微镜
DOI：
10.1117/12.2550682
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Görlitz F
中科院分区：
文献类型：
--
作者：
Görlitz F
超分辨显微镜技术已经克服了衍射极限，提供了接近荧光标记的图像分辨率。然而，许多这些技术需要大量的实验资源和专业知识，并且需要很长的图像数据采集时间，因此很难从足够大的样本数中获取超分辨率数据来克服内在的生物变异。我们已经努力使受激发射损耗（STED）显微镜和单分子定位显微镜（SMLM）更容易实现，更实用，可以对大量细胞进行成像。在这里，我们介绍了正在进行的开发easylm STED和easySTORM的工作，包括一个新的模块化显微镜框架，我们相信它可以使显微镜技术的原型更容易，并在资源较低的环境中实现和维护它们。
Super-resolved microscopy techniques have overcome the diffraction limit to provide image resolutions approaching the scale of fluorescent labels. However, many of these techniques require significant experimental resources and expertise and impose long image data acquisition times, making it difficult to acquire super-resolved data from sufficiently large sample numbers to overcome intrinsic biological variation. We have worked to make stimulated emission depletion (STED) microscopy and single molecule localisation microscopy (SMLM) more straightforward to implement and more practical to image larger numbers of cells. Here we present work in progress developing easySLM STED and easySTORM, including a new modular microscope frame that we believe can make it easier to prototype microscopy techniques and to implement and maintain them in lower resourced settings.
DOI：
10.1073/pnas.0406877102
发表时间：
2005-09-13
影响因子：
11.1
作者：
Gustafsson, MGL
通讯作者：
Gustafsson, MGL
影响因子：
2.9
作者：
Holm, Thorge;Klein, Teresa;Sauer, Markus
通讯作者：
Sauer, Markus