Cell biology of mating in Candida albicans
Cell biology of mating in Candida albicans
复制标题
DOI：
10.1128/ec.2.1.49-61.2003
复制
发表时间：
2003-02-01
期刊：
影响因子：
--
通讯作者：
Soll, DR
中科院分区：
文献类型：
--
作者：
Lockhart, SR;Daniels, KJ;Soll, DR
最近证实，白色念珠菌的交配型样基因座MTLa和MTLalpha的任一个纯合菌株经历白色不透明转换，并且不透明相表型的表达极大地增强了菌株之间的交配。利用后者的属性，以获得高频率的交配，我们的特点是细胞生物学的交配过程中的C。白色念珠菌。采用连续视频显微镜，计算机辅助三维重建的活细胞，荧光显微镜，我们监测了交配相关的过程中的接合，管的形成，融合，出芽，隔膜的形成，和子细胞的发展和空间和时间的动态核迁移和分裂。根据这些观察，提出了C.白色念珠菌交配被制定。这些阶段包括shmooing，接合管的趋化性，管融合和核联合，接合桥中的液泡扩张和核分离，合子中的异步核分裂，芽生长，核迁移到子细胞中，分隔和子细胞出芽。由于没有细胞学上的核配现象，因此进行了遗传实验以评估标记分离。没有观察到交配，这表明交配发生在自然发生的纯合子a和a菌株之间没有核配体的情况下。本研究首次描述了中华绒螯蟹交配过程的细胞生物学特征。白色念珠菌
It was recently demonstrated that strains homozygous for either of the mating type-like loci MTLa and MTLalpha of Candida albicans undergo white-opaque switching and that expression of the opaque-phase phenotype greatly enhances mating between strains. Exploiting the latter property to obtain high-frequency mating, we have characterized the cell biology of the mating process of C. albicans. Employing continuous videomicroscopy, computer-assisted three-dimensional reconstruction of living cells, and fluorescence microscopy, we have monitored the mating-associated processes of conjugation, tube formation, fusion, budding, septum formation, and daughter cell development and the spatial and temporal dynamics of nuclear migration and division. From these observations, a model for the stages in C. albicans mating is formulated. The stages include shmooing, chemotropism of conjugation tubes, fusion of tubes and nuclear association, vacuole expansion and nuclear separation in the conjugation bridge, asynchronous nuclear division in the zygote, bud growth, nuclear migration into the daughter cell, septation, and daughter cell budding. Since there was no cytological indication of karyogamy, genetic experiments were performed to assess marker segregation. Recombination was not observed, suggesting that mating takes place in the absence of karyogamy between naturally occurring, homozygous a and a strains. This study provides the first description of the cell biology of the mating process of C. albicans.