THE USE OF GLUTARALDEHYDE AND TANNIC-ACID TO PRESERVE RECONSTITUTED COLLAGEN FOR ELECTRON-MICROSCOPY
THE USE OF GLUTARALDEHYDE AND TANNIC-ACID TO PRESERVE RECONSTITUTED COLLAGEN FOR ELECTRON-MICROSCOPY
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DOI：
10.1007/bf00493137
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
MEEK, KM
中科院分区：
文献类型：
--
作者：
MEEK, KM
The effects of glutaraldehyde and tannic acid on the axial periodicity of collagen were measured. Both fixatives produce axial shrinkage of the collagen but whereas glutaraldehyde produces 7% shrinkage, tannic acid produces only 2% shrinkage. The technique of C/Pt shadowing was used to estimate the extent to which the collagen fibrils flatten down when they are dried onto grids for EM without prior embedding and sectioning. The influence of fixation was studied and minimum distortion occurred when both tannic acid and glutaraldehyde were used to preserve the protein structure.
