Mapping an origin of DNA replication at a single-copy locus in exponentially proliferating mammalian cells
Mapping an origin of DNA replication at a single-copy locus in exponentially proliferating mammalian cells
复制标题
绘制呈指数增殖的哺乳动物细胞中单拷贝基因座 DNA 复制起点的图谱
DOI：
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复制
发表时间：
1990
影响因子：
5.3
通讯作者：
Melvin L. DePamphilis
中科院分区：
文献类型：
--
作者：
Lyubomir T. Vassilev;W. Burhans;Melvin L. DePamphilis
最近已经开发了一种用于确定双向DNA复制起点的物理位置的通用方法，并且显示出能够正确地鉴定猿猴病毒40复制起点（L. Vassilev和E. M.约翰逊，核酸研究17：7693-7705，1989）。与以前报道的其他方法相比，这种方法的优点是它避免了使用代谢抑制剂，不需要细胞同步化，也不需要原始序列的多个拷贝。应用这种方法，以指数增长的中国仓鼠卵巢细胞含有nonamplified，单拷贝二氢叶酸还原酶基因位点显示，DNA复制开始双向的起始区约2.5个酶长约17个酶的DHFR基因下游为中心，与先前描述的早期复制序列相一致。这些结果证明了该作图方案用于鉴定细胞复制起点的实用性，并表明在正常和扩增的DHFR基因座中使用相同的细胞起点。
A general method for determining the physical location of an origin of bidirectional DNA replication has been developed recently and shown to be capable of correctly identifying the simian virus 40 origin of replication (L. Vassilev and E. M. Johnson, Nucleic Acids Res. 17:7693-7705, 1989). The advantage of this method over others previously reported is that it avoids the use of metabolic inhibitors, the requirement for cell synchronization, and the need for multiple copies of the origin sequence. Application of this method to exponentially growing Chinese hamster ovary cells containing the nonamplified, single-copy dihydrofolate reductase gene locus revealed that DNA replication begins bidirectionally in an initiation zone approximately 2.5 kilobases long centered about 17 kilobases downstream of the DHFR gene, coinciding with previously described early replicating sequences. These results demonstrate the utility of this mapping protocol for identifying cellular origins of replication and suggest that the same cellular origin is used in both the normal and the amplified DHFR locus.
真核 DNA 复制起源研究的新起点。
DOI：
10.1016/0167-4781(89)90123-1
发表时间：
1989
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Umek,RM;Linskens,MH;Kowalski,D;Huberman,JA
通讯作者：
Huberman,JA
扩增的二氢叶酸还原酶复制子起始区的分子内 DNA 三链体、弯曲 DNA 和 DNA 解旋元件。
影响因子：
5.6
作者：
Caddle,MS;Lussier,RH;Heintz,NH
通讯作者：
Heintz,NH