5'-DEOXY-5'-METHYLTHIOADENOSINE INHIBITION OF RAT LYMPHOCYTE-T PHOSPHODIESTERASE - CORRELATION WITH INHIBITION OF CON-A INDUCED PROLIFERATION
5'-DEOXY-5'-METHYLTHIOADENOSINE INHIBITION OF RAT LYMPHOCYTE-T PHOSPHODIESTERASE - CORRELATION WITH INHIBITION OF CON-A INDUCED PROLIFERATION
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DOI：
10.1016/0006-291x(83)91743-6
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
PERIGNON, JL
中科院分区：
文献类型：
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作者：
CHRISTA, L;THUILLIER, L;PERIGNON, JL
5“-脱氧-5”-甲硫基腺苷[MTA]以剂量依赖性方式（50-1000 μ M）抑制伴刀豆球蛋白A诱导的大鼠T淋巴细胞增殖。当MTA与伴刀豆球蛋白A同时加入细胞时，MTA对淋巴细胞增殖的抑制程度最大。从30分钟到第6小时的cAMP水平的测定表明，5“-脱氧-5”-甲硫基腺苷抑制与在该有丝分裂原识别阶段的cAMP升高相关。抑制大鼠T淋巴细胞增殖的5“-脱氧-5”-甲硫基腺苷浓度也抑制磷酸二酯酶活性。这种生化机制可能对5“-脱氧-5”-甲硫基腺苷抑制是特异性的，因为在另一种大鼠T淋巴细胞增殖抑制模型（2“-脱氧腺苷10 μ M，在腺苷脱氨酶缺乏条件下：2”-脱氧共形霉素10 μ M）中，没有证实cAMP水平的显著改变。
5''-Deoxy-5''-methylthioadenosine [MTA] inhibits concanavalin A induced rat T lymphocyte proliferation in a dose dependent manner (50-1000 .mu.M). The extent of inhibition by MTA of lymphocyte proliferation was greatest when MTA was added to the cells at the same time as concanavalin A. The determination of cAMP level from 30 min to the 6th h shows that 5''-Deoxy-5''-methylthioadenosine inhibition is correlated with an elevation of cAMP at this mitogen recognition phase. 5''-Deoxy-5''-methylthioadenosine concentrations that inhibit rat T lymphocyte proliferation also inhibit phosphodiesterase activity. This biochemical mechanism could be specific to 5''-Deoxy-5''-methylthioadenosine inhibition since in an another model of inhibition of rat T lymphocyte proliferation (2''-deoxyadenosine 10 .mu.M, in adenosine deaminase deficiency conditions: 2''-deoxycoformycin 10 .mu.M), no significant modification of cAMP level can be demonstrated.