LT(R192G), a non-toxic mutant of the heat-labile enterotoxin of Escherichia coli, elicits enhanced humoral and cellular immune responses associated with protection against lethal oral challenge with Salmonella spp.
LT(R192G), a non-toxic mutant of the heat-labile enterotoxin of Escherichia coli, elicits enhanced humoral and cellular immune responses associated with protection against lethal oral challenge with Salmonella spp.
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DOI：
10.1016/s0264-410x(97)00255-7
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发表时间：
1998-04-01
期刊：
影响因子：
5.5
通讯作者：
Clements, JD
中科院分区：
文献类型：
--
作者：
Chong, C;Friberg, M;Clements, JD
在目前的研究中，我们检测了一种新的粘膜佐剂LT(R192G)增强针对沙门氏菌灭活的体液和细胞免疫反应的能力。并对野生型生物的致命口腔攻击起到保护作用。口服灭活都柏林沙门氏菌与LT(R192G)联合免疫的小鼠可抵抗致死性口服攻击，并能产生较高的干扰素-γ、IL-2和免疫球蛋白G反应。本研究表明LT(R192G)对伤寒类疾病的保护作用是上调/增强Th1臂的免疫应答。当作为粘膜佐剂使用时，LT(R192G)能够通过增强对这些生物的整体体液和细胞宿主免疫反应，特别是免疫反应的Th1臂，使灭活的细菌或病毒能够用作疫苗。这些发现对疫苗开发具有重要意义，并进一步支持LT(R192G)作为一种安全、有效的粘膜接种疫苗佐剂的潜力。(C)1998爱思唯尔科学有限公司。所有晚上已预订。
In the current study we examined the ability of a novel mucosal adjuvant, LT(R192G), to enhance the humoral and cellular immune responses against killed Salmonella spp. and to affect protection against lethal oral challenge with wild-type organisms. Mice orally immunized with killed S. dublin in conjunction with LT(R192G) were protected against lethal oral challenge and had higher IFN-gamma IL-2 and IgG responses than did mice orally immunized with killed S. dublin alone which were not protected This study demonstrates that the function of LT(R192G) in protection against typhoid-like disease is to upregulate/enhance the Th1 arm of the immune response against killed organisms. When used as a mucosal adjuvant, LT(R192G) enables the use of killed bacteria or viruses as vaccines by enhancing the overall humoral and cellular host immune response to these organisms, especially the Th1 arm of the immune response. These findings have significant implications for vaccine development and further support the potential of LT(R192G) to function as a safe, effective adjuvant for mucosally administered vaccines. (C) 1998 Elsevier Science Ltd. All nights reserved.