Cloning, expression prolife, and immune characterization of a novel stat family member (stat5bl) in Chinese tongue sole (Cynoglossus semilaevis)
Cloning, expression prolife, and immune characterization of a novel stat family member (stat5bl) in Chinese tongue sole (Cynoglossus semilaevis)
复制标题
中华舌鳎 (Cynoglossus semilaevis) 新型 stat 家族成员 (stat5bl) 的克隆、表达增殖和免疫特征
DOI：
10.1016/j.fsi.2018.10.030
复制
发表时间：
2019
影响因子：
4.7
通讯作者：
Chen Song lin
中科院分区：
文献类型：
--
作者：
Wei Min;Xu Wen teng;Gan Tian;Wang Lei;Zhang Hong xiang;Zhao Fa zhen;Chen Song lin
STAT在JAK/STAT信号通路中在先天免疫中发挥重要作用，其中STAT5由于在鱼类和哺乳动物中存在重复形式而受到特别关注。在中国鳎目鱼中，经QTL筛选，stat5bl1可能是与抗性有关的候选菌株。本研究克隆了stat5blcdna的全长，并分析了其表达模式。stat5bl1主要在免疫组织中表达，其中肝脏表达量最高，其次是皮肤和鳃。在肝、皮肤、鳃、肾、肠、脾等6个组织中检测v后的表达谱。harveyiinfection。stat5bl1可以被v上调。除肝脏外，各组织均有哈维氏感染，但峰值时间不同。相反，stat5bl1在肝脏中显著下调。为了阐明stat5在肝脏中的作用，我们利用原代肝细胞培养进行了体外rna检测。敲低stat5bl1可以调控与JAK/STAT通路密切相关的基因的表达。本研究将扩大我们对stat5blin鱼类免疫的认识。
STAT plays important roles in innate immunity during JAK/STAT signaling pathway, and STAT5 is particularly focused due to the existence of duplicated forms in fish and mammal. In Chinese tongue sole,stat5blwas suggested to be a candidate related toVibrio harveyiresistance based on previous QTL screening. In this study, the full length ofstat5blcDNA was cloned and its expression patterns were analyzed.stat5blwas predominantly expressed in immune tissues, where the highest level was observed in liver, followed by skin and gill. Time course expression patterns were examined in six tissues (liver, skin, gill, kidney, intestine, spleen) afterV. harveyiinfection.stat5blcould be up-regulated byV. harveyiinfection in all tissues except liver, despite the timepoints of peak were different. In contrast,stat5blwas significantly downregulated in liver. To elucidate the role ofstat5blin liver,in vitroRNAi were performed using primary liver cell culture. Knockdown ofstat5blcould regulate the expression of genes closely related to JAK/STAT pathway. This study would enlarge our understanding ofstat5blin fish immunity.