CYTOLYSIS BY H-2-SPECIFIC-T KILLER CELLS - ASSEMBLY OF TUBULAR COMPLEXES ON TARGET MEMBRANES
CYTOLYSIS BY H-2-SPECIFIC-T KILLER CELLS - ASSEMBLY OF TUBULAR COMPLEXES ON TARGET MEMBRANES
复制标题
DOI：
10.1084/jem.157.5.1483
复制
发表时间：
1983-01-01
影响因子：
15.3
通讯作者：
PODACK, ER
中科院分区：
文献类型：
--
作者：
DENNERT, G;PODACK, ER
从单向混合淋巴细胞反应中克隆的小鼠T杀伤细胞的特征在于其Lyt表型和特异性。选择两个识别H-2Dd的Lyt-1-2+表型的克隆，并通过负染色和薄切片EM检查它们的细胞溶解功能。当与H-2d靶标S194孵育时，它们组装2种类型的管状复合物，即多穿孔素1和2。这两种结构似乎是由可能起源于T杀伤细胞的致密颗粒和/或高尔基体的前体聚合而产生的。多孔蛋白似乎与在裂解反应期间释放并转移到靶膜的囊泡相关，如免疫电子显微镜所示。由于靶细胞裂解与靶细胞膜上多穿孔素的出现密切相关，因此认为多穿孔素参与了细胞介导的杀伤反应。虽然多穿孔素的大小和几个分子特性与补体不同，但这些ciruclar复合物和多穿孔素（C9）之间存在惊人的相似性;它们可能属于一个密切相关的溶细胞效应分子家族。
Cloned mouse T killer cells derived from one-way mixed lymphocyte reactions were characterized with regard to their Lyt phenotype and specificity. Two clones of Lyt-1-2+ phenotype that recognized H-2Dd were selected and examined for their cytolytic function by negative staining and thin section EM. When incubated with the H-2d target S194, they assemble 2 types of tubular complexes, polyperforin 1 and 2. Both structures appear to arise by polymerization of precursors that may originate in dense granules and/or Golgi of T killer cells. Polyperforins appear to be associated with vesicles that are released during the lytic reaction and transferred to target membranes as shown by immunoelectron microscopy. Since there is a close correlation between target lysis and the appearance of polyperforins on target membranes, it is suggested that polyperforins take part in the cell-mediated killing reaction. Although polyperforins are different in size and several molecular properties from complement, there are striking similarities between these ciruclar complexes and polyperforin (C9); they may belong to a closely related family of cytolytic effector molecules.