Anti-IL-33 Antibody Has a Therapeutic Effect in an Atopic Dermatitis Murine Model Induced by 2, 4-Dinitrochlorobenzene
Anti-IL-33 Antibody Has a Therapeutic Effect in an Atopic Dermatitis Murine Model Induced by 2, 4-Dinitrochlorobenzene
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DOI：
10.1007/s10753-017-0673-7
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发表时间：
2018-02-01
期刊：
影响因子：
5.1
通讯作者：
Han, Xiuping
中科院分区：
文献类型：
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作者：
Peng, Ge;Mu, Zhenzhen;Han, Xiuping
IL-33是IL-1家族的新成员，在过敏性疾病中发挥作用。本研究采用2，4-二硝基氯苯(DNCB)诱导的特应性皮炎(AD)小鼠模型，评价了抗鼠IL-33抗体(αIL-33Ab)对AD的抑制作用。从第1天到第33天，每组小鼠皮下注射1小时后的αIL-33Ab，共14次。对照组接受他克莫司治疗。比较两组患者的皮肤损伤和抓挠行为。并分析耳厚、皮炎评分、嗜酸性粒细胞和肥大细胞浸润及血清IgE水平。并探讨了血清IL-33和可溶性(S)sT_2与AD疾病活动指数的相关性。用α-IL-33Ab治疗DNCB诱导的类AD小鼠后，类AD症状得到改善。αIL-33Ab还能显著降低小鼠外周血嗜酸性粒细胞和肥大细胞的浸润，降低血清IgE水平。本研究提示阻断IL-33对阿尔茨海默病有一定疗效。
IL-33 is a new member of the IL-1 family that plays a role in allergic disease. In this study, we evaluated the potential on the inhibition of atopic dermatitis (AD) of anti-mouse IL-33 antibody (alpha IL-33Ab) using 2, 4-dinitrochlorobenzene (DNCB)-induced AD mice model. We treated mice with alpha IL-33Ab via subcutaneous injection of each DNCB treatment 1 h later from day 1 to day 33 for 14 times. A control group received tacrolimus. Skin lesion and scratching behavior were compared. Ear thickness, dermatitis score, eosinophils and mast cells infiltration, and serum IgE levels were also analyzed. Correlations between serum IL-33 as well as soluble(s) ST2 and AD disease activity index in human AD were also investigated. DNCB-induced AD-like mice treated with alpha IL-33Ab showed improved AD-like symptoms. Eosinophils and mast cells infiltration and serum IgE levels were also significantly reduced by alpha IL-33Ab. Our study suggests that blockade of IL-33 has a curative effect on AD.