CHARACTERIZATION OF 4 MERKEL CELL-CARCINOMA ADHERENT CELL-LINES
CHARACTERIZATION OF 4 MERKEL CELL-CARCINOMA ADHERENT CELL-LINES
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DOI：
10.1002/ijc.2910600115
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发表时间：
1995-01-03
影响因子：
6.4
通讯作者：
BELL, JR
中科院分区：
文献类型：
--
作者：
LEONARD, JH;DASH, P;BELL, JR
我们之前已经描述了从默克尔细胞癌（MCC）（也称为皮肤小细胞癌或皮肤神经内分泌癌）建立的许多细胞系。这些细胞均以悬浮培养物形式生长，其形态和生长特征与小细胞肺癌 (SCLC) 系 1、2 和 3 型相似。我们现在报告了另外 4 种 MCC 细胞系，它们与 SCLC 4 型细胞系相似，因为它们以贴壁单层形式生长。这些MCC系属于变体亚组，因为它们不再表达大多数神经内分泌标记物，以低细胞密度生长，并且群体倍增时间为1-5天，而MCC悬浮系的倍增时间为6-12天。 MCC14/1 和 MCC14/2 是从同一转移节点建立的，似乎代表了形态、组织化学标记物和 DNA 含量不同的肿瘤的 2 个克隆。我们详细介绍了这 4 个细胞系的形态、DNA 含量和免疫组织化学，并将它们的生长模式与悬浮生长的 SCLC 和 MCC 细胞系进行了比较。 (C) 1995 Wiley-Liss, Inc.
We have previously described the establishment of a number of cell lines from Merkel cell carcinoma (MCC), also known as small cell cancer of the skin or neuroendocrine carcinoma of the skin. These cells, all of which grew as suspension cultures, were found to resemble small cell lung cancer (SCLC) lines types 1, 2 and 3 by their morphology and growth characteristics. We now report 4 more MCC cell lines which resemble the SCLC type 4 cell lines in that they grow as adherent monolayers. These MCC lines would belong to the variant subgroup as they no longer express most neuroendocrine markers, grow at low cell density and have population doubling times of 1-5 days in contrast to the MCC suspension lines which have doubling times of 6-12 days. MCC14/1 and MCC14/2 were established from the same metastatic node and would appear to represent 2 clones of the tumour which differ in morphology, histochemical markers and DNA content. We present details of the morphology, DNA content and immunohistochemistry of these 4 lines and compare their growth patterns with those of SCLC and MCC lines which grow in suspension. (C) 1995 Wiley-Liss, Inc.