Leonardo, a Drosophila 14-3-3 protein involved in learning, regulates presynaptic function
Leonardo, a Drosophila 14-3-3 protein involved in learning, regulates presynaptic function
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DOI：
10.1016/s0896-6273(00)80948-4
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发表时间：
1997-08-01
期刊：
影响因子：
16.2
通讯作者：
Davis, RL
中科院分区：
文献类型：
--
作者：
Broadie, K;Rushton, E;Davis, RL
列奥纳多基因编码14-3-3蛋白家族的一个保守成员，该蛋白家族在果蝇群体中起作用。免疫定位的蛋白质表明，它表达在突触连接和丰富的神经肌肉接头（NMJ）的突触前扣。无效的列奥纳多突变体在成熟胚胎时死亡。突变体NMJ的电生理学测定表明，基础突触传递减少了30%，并且在升高的刺激频率和低外部[Ca 2 +]下，传递振幅、保真度和抗疲劳性能降低。此外，传输增强和强直后增强（PTP）在突变体中被破坏。这些结果表明，列奥纳多在调节突触囊泡动力学中发挥作用，这一功能可能是突触调制特性的基础，从而使学习。
The leonardo gene encodes a conserved member of the 14-3-3 protein family, which plays a role in Drosophila teaming. Immunological localization of the protein shows that it is expressed at synaptic connections and enriched in presynaptic boutons of the neuromuscular junction (NMJ). Null leonardo mutants die as mature embryos. Electrophysiological assays of the mutant NMJ demonstrate that basal synaptic transmission is reduced by 30% and that transmission amplitude, fidelity, and fatigue resistance properties are reduced at elevated stimulation frequencies and in low external [Ca2+]. Moreover, transmission augmentation and post-tetanic potentiation (PTP) are disrupted in the mutant. These results suggest that Leonardo plays a role in the regulation of synaptic vesicle dynamics, a function which may underlie synaptic modulation properties enabling learning.