Endothelial renin-angiotensin pathway: evidence for intracellular synthesis and secretion of angiotensins.
Endothelial renin-angiotensin pathway: evidence for intracellular synthesis and secretion of angiotensins.
复制标题
内皮肾素-血管紧张素途径：血管紧张素细胞内合成和分泌的证据。
DOI：
10.1161/01.res.60.3.422
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发表时间：
1987
影响因子：
20.1
通讯作者：
Dzau,VJ
中科院分区：
文献类型：
--
作者：
Kifor,I;Dzau,VJ
培养的牛主动脉内皮细胞(BAEC)含有肾素和血管紧张素原。为了检验血管紧张素是否在细胞内合成和分泌，我们检测了细胞提取液和完整融合的BAEC的无血清培养液。血管紧张素在反相高效液相色谱和直接放射免疫法上的保留时间被鉴定。血管内皮细胞及其培养液中含有血管紧张素Ⅱ和血管紧张素Ⅲ。血管紧张素Ⅱ和血管紧张素Ⅲ在培养液中的积聚速率是时间的非线性函数，最高速率出现在前15分钟。30分钟内血管紧张素Ⅱ的积累量超过胞内浓度的200%，血管紧张素Ⅲ的积累量超过初始胞内浓度的500%。培养液中未检测到肾素和血管紧张素原。这些细胞的活力是由培养液中的低乳酸脱氢酶活性(低于细胞水平的0.5%)支持的。这些数据表明，BAEC具有合成和分泌血管紧张素的能力。我们推测，这种内皮来源的血管紧张素系统可能在影响局部血管张力的过程中发挥重要的旁分泌或自分泌作用。
Cultured bovine aortic endothelial cells (BAEC) contain renin and angiotensinogen. To examine whether angiotensins are synthesized intracellularly and secreted by these cells, we assayed cell extracts as well as serum-free media of intact confluent BAEC. Angiotensins were identified by their retention time on reverse phase high performance liquid chromatography and direct radioimmunoassay. BAEC and their media contain angiotensin II and angiotensin III. The rate of angiotensin accumulation in the media was a nonlinear function of time; the highest rate occurred in the first 15 minutes. The amount of angiotensin II accumulated in 30 minutes exceeded 200% of the intracellular concentration and that of angiotensin III exceeded 500% of the initial intracellular content. Neither renin nor angiotensinogen could be detected in the media. The viability of these cells was supported by low lactic dehydrogenase activity in the media (less than 0.5% of cellular level). These data suggest that BAEC is capable of synthesizing and secreting angiotensins. We postulate that this endothelial-derived angiotensin system may play an important paracrine or autocrine role in influencing local vascular tone.