Mini-Review AAA Proteins: Lords of the Ring
Mini-Review AAA Proteins: Lords of the Ring
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发表时间：
2000
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通讯作者：
R. Vale
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作者：
R. Vale
AAA ATP酶（与各种细胞活动相关的酶）1在许多细胞活动中发挥重要作用，包括蛋白水解、蛋白折叠、膜运输、细胞骨架调节、细胞器生物合成、DNA复制和细胞内运动。最近的结构和酶的研究提供了线索的性质保守的AAA结构域，定义了这个大的蛋白质超家族。在许多情况下，AAA结构域组装成六聚体环，其可能在ATP酶循环期间改变其形状。这种核苷酸依赖性构象转换可以向结合的蛋白质施加张力，从而允许AAA蛋白质展开多肽，解离蛋白质-蛋白质相互作用，或产生沿轨道的单向运动沿着。因此，AAA蛋白可能代表了广泛的一类机械酶，它们在许多不同的生物环境中进化出了使用基本相似的构象变化的独特方式。
AAA ATPases (those associated with various cellular activities) 1 play important roles in numerous cellular activities including proteolysis, protein folding, membrane trafficking, cytoskeletal regulation, organelle biogenesis, DNA replication, and intracellular motility. Recent structural and enzymatic studies are providing clues into the proper-ties of the conserved AAA domain that defines this large protein superfamily. In many cases, AAA domains assem-ble into hexamic rings that are likely to change their shape during the ATPase cycle. This nucleotide-dependent conformational switch may apply tension to bound proteins and thereby allow AAA proteins to unfold polypeptides, dissociate protein–protein interactions, or generate unidirectional motion along a track. Thus, AAA proteins may represent a broad class of mechanoenzymes that have evolved unique ways of using a fundamentally similar conformational change in many different biological settings.