A bone-like nano-hydroxyapatite/collagen loaded injectable scaffold
A bone-like nano-hydroxyapatite/collagen loaded injectable scaffold
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一种骨状纳米羟基磷灰石/胶原蛋白负载的可注射支架
DOI：
10.1088/1748-6041/4/5/055005
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发表时间：
2009-10-01
影响因子：
4
通讯作者：
Feng, Qingling
中科院分区：
文献类型：
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作者：
Huang, Zhi;Tian, Jing;Feng, Qingling
从临床的角度来看，使用可注射支架是非常有吸引力的，因为它最大限度地减少了患者的不适，感染的风险，疤痕形成和治疗成本。骨是指由矿化的胶原纤维构成的材料家族。本研究的主要目的是制备一种纳米羟基磷灰石/胶原（nHAC）负载壳聚糖（C）/β-甘油磷酸（GP）的类骨可注射支架。证明了在nHAC存在下开发温敏性和可注射壳聚糖溶液的可行性。基于细胞计数试剂盒-8（CCK-8）测定，使用骨髓源性间充质干细胞（MSC）来测量C/GP/nHAC支架的细胞增殖。结果表明，MSCs在C/GP/nHAC复合支架上增殖正常。本研究所开发的C/GP/nHAC复合支架具有良好的可注射性、热不可逆性和温和的固化条件。需要不超过0.02 g ml-1的nHAC填料来形成非衰变水凝胶。
From a clinical perspective, the use of injectable scaffolds is very attractive as it minimizes patient discomfort, risk of infection, scar formation and the cost of treatment. Bone refers to a family of materials that are constructed by mineralized collagen fibrils. The main objective of this research was to develop a bone-like nano-hydroxyapatite/collagen (nHAC) loaded chitosan (C)/β-glycerophosphate (GP) injectable scaffold. The feasibility of developing a thermo-sensitive and injectable chitosan solution in the presence of nHAC was demonstrated. Bone-marrow-derived messenchymal stem cells (MSCs) were used to measure the cell proliferation of C/GP/nHAC scaffolds based on the cell count kit-8 (CCK-8) assay. It was found that MSCs proliferated normally with the C/GP/nHAC composite scaffolds. The C/GP/nHAC composite scaffolds developed in this study exhibited good injectability, thermo-irreversible properties and solidified under mild conditions. No more than 0.02 g ml−1 of nHAC filler was required to form a non-decaying hydrogel.