CRISPR Typing and Antibiotic Resistance Correlates with Polyphyletic Distribution in Human Isolates of Salmonella Kentucky.
CRISPR Typing and Antibiotic Resistance Correlates with Polyphyletic Distribution in Human Isolates of Salmonella Kentucky.
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CRISPR 分型和抗生素耐药性与肯塔基州沙门氏菌人类分离株的多系分布相关。
DOI：
10.1089/fpd.2017.2298
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发表时间：
2018
影响因子：
2.8
通讯作者：
Shariat,NikkiW
中科院分区：
文献类型：
--
作者：
Vosik,Dorothy;Tewari,Deepanker;Dettinger,Lisa;M'ikanatha,NkuchiaM;Shariat,NikkiW
虽然很少与报告的人类沙门氏菌病相关，但肠道沙门氏菌肯塔基州亚种（肯塔基州血清型）是美国报告的最常见的非临床、非人血清型。本研究的目的是使用重复的规则间隔短回文重复序列（CRISPR）-多毒力位点序列分型（MVLST）对提交给宾夕法尼亚州卫生部的肯塔基州血清的人类临床分离株集合进行分型，并确定这些菌株的抗生素耐药性程度。该分析突出了肯塔基州血清的多系性，并将我们的分离物分为两组，组I和组II，它们在我们的收集物中具有相同的代表性。此外，使用国家抗生素耐药性监测系统（NARMS）抗生素组对所有分离株进行的抗菌药物敏感性测试表明，耐药性特征可分为两组。第一组菌株耐头孢烯类和青霉素类，而第二组菌株耐喹诺酮类，庆大霉素，磺胺异恶唑。总体而言，50%的分离株对三类或三类以上抗生素耐药，30%对五类或五类以上抗生素耐药。抗生素耐药性与两个不同谱系的相关性可能反映了肯塔基州血清的两个不同储存库内的适应性，以及对抗生素的不同暴露。
Although infrequently associated with reported salmonellosis in humans,Salmonella enterica, subsp.entericaserovar Kentucky (ser. Kentucky) is the most common nonclinical, nonhuman serovar reported in the United States. The goal of this study was to use Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–multi-virulence-locus sequence typing (MVLST) to subtype a collection of human clinical isolates of ser. Kentucky submitted to the Pennsylvania Department of Health and to determine the extent of antibiotic resistance in these strains. This analysis highlighted the polyphyletic nature of ser. Kentucky, and separated our isolates into two groups, Group I and Group II, which were equally represented in our collection. Furthermore, antimicrobial susceptibility testing on all isolates using a National Antimicrobial Resistance Monitoring System (NARMS) panel of antibiotics demonstrated that resistance profiles could be divided into two groups. Group I isolates were resistant to cephems and penicillins, whereas Group II isolates were resistant to quinolones, gentamicin, and sulfisoxazole. Collectively, 50% of isolates were resistant to three or more classes of antibiotics and 30% were resistant to five or more classes. The correlation of antibiotic resistance with the two different lineages may reflect adaptation within two distinct reservoirs of ser. Kentucky, with differential exposure to antimicrobials.