Methodological approaches to understand the molecular mechanism of structural plasticity of dendritic spines
Methodological approaches to understand the molecular mechanism of structural plasticity of dendritic spines
复制标题
DOI：
10.1111/ejn.14734
复制
发表时间：
2020-04
影响因子：
3.4
通讯作者：
T. Mikuni;M. Uchigashima
中科院分区：
文献类型：
--
作者：
T. Mikuni;M. Uchigashima
树突棘是从神经元树突发出的微小突起，通常容纳单个兴奋性突触后。树突棘的结构可塑性被认为是突触功能可塑性和学习记忆过程中神经回路重组的关键。棘的结构可塑性是由复杂的生化信号与各种空间和时间尺度介导的。基于药理学、遗传学、分子学、成像和光学方法的各种方法已经被开发并应用于剖析复杂的信号转导途径。在这篇综述中，我们概述了传统的和新的方法来识别，监测和操纵树突棘的结构可塑性的关键分子，最终旨在了解行为动物学习和记忆的分子机制。
Dendritic spines are tiny protrusions emanating from the neuronal dendrites, typically housing single excitatory postsynapses. Structural plasticity of dendritic spines is considered to be essential for synaptic functional plasticity and also reorganization of neural circuits during learning and memory. Structural plasticity of spines is mediated by complex biochemical signaling with various spatial and temporal scales. A variety of methods based on pharmacological, genetic, molecular, imaging and optical approaches has been developed and applied to dissect the complex signal transduction pathways. In this review, we overview both conventional and new methodological approaches to identify, monitor and manipulate key molecules for structural plasticity of dendritic spines, ultimately aiming to understand the molecular mechanism of learning and memory in behaving animals.