Mapping and evaluation of Malus xdomestica microsatellites in apple and pear
Mapping and evaluation of Malus xdomestica microsatellites in apple and pear
复制标题
苹果和梨中 Malus xdomestica 微卫星的测绘与评估
DOI：
10.21273/jashs.128.4.0515
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发表时间：
2003-07-01
影响因子：
1.9
通讯作者：
Brown, SK
中科院分区：
文献类型：
--
作者：
Hemmat, M;Weeden, NF;Brown, SK
我们绘制了41套简单重复序列（SSR）引物，在四个实验室独立开发的DNA多态性。所有引物组都给出了多态性，这些多态性可以定位在我们的苹果[Malus sylveshis（L.）Mill.变种Artistica（Borkh.）Mansf.]。利用SSR引物对“Wijcik McIntosh”、NY 75441-58、“Golden Delicious”和“Liberty”等4个已构建了较为完整的连锁图谱的品种进行了连锁群鉴定。在一些情况下，两个或更多的SSR是同线的，除了一个明显的易位涉及连锁群（LG）6，这些联系是保守的整个六个地图。24个SSR引物表现出一致的多态性，可作为苹果遗传图谱的标准锚标记。梨（Pyrus communis L.）群体中的SSR标记表明，许多苹果SSR标记将是有用的梨定位。然而，有些引物在梨中产生的片段大小与苹果中的片段大小存在显着差异。
We mapped DNA polymorphisms generated by 41 sets of Simple Sequence Repeat (SSR) primers, developed independently in four laboratories. All primer sets gave polymorphisms that could be located on our 'White Angel' x 'Rome Beauty' map for apple [Malus sylveshis (L.) Mill. Var. domestica (Borkh.) Mansf.]. The SSR primers were used to identify homologous linkage groups in 'Wijcik McIntosh', NY 75441-58, 'Golden Delicious', and 'Liberty' cultivars for which relatively complete linkage maps have been constructed from isozyme and Random Amplified Polymorphic DNA (RAPD) markers. In several instances, two or more SSRs were syntenic, and except for an apparent translocation involving linkage group (LG) 6, these linkages were conserved throughout the six maps. Twenty-four SSR primers were consistently polymorphic, and these are recommended as standard anchor markers for apple maps. Experiments on a pear (Pyrus communis L.) population indicated that many of the apple SSRs would be useful for mapping in pear. However some of the primers produced fragments in pear significantly different in size than those in apple.