fgf8 mRNA decay establishes a gradient that couples axial elongation to patterning in the vertebrate embryo
fgf8 mRNA decay establishes a gradient that couples axial elongation to patterning in the vertebrate embryo
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DOI：
10.1038/nature02216
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发表时间：
2004-01-29
期刊：
影响因子：
64.8
通讯作者：
Pourquié, O
中科院分区：
文献类型：
--
作者：
Dubrulle, J;Pourquié, O
脊椎动物胚胎的形成和图案化是以头到尾的顺序发生的。这种从胚胎后端开始的身体形成的渐进模式需要组织分化的严格时间协调-这是一个涉及成纤维细胞生长因子（FGF）信号传导的过程。在这里，我们发现fgf 8信使RNA的转录仅限于胚胎的后尖生长。fgf 8 mRNA在新形成的组织中逐渐降解，导致在胚胎后部形成mRNA梯度。该fgf 8 mRNA梯度被翻译成FGF 8蛋白的梯度，其与激酶Akt（FGF信号传导的下游效应物）的分级磷酸化相关。这种机制提供了一种有效的手段来监测FGF信号传导的时间，将胚胎组织的分化与胚胎的后部伸长相结合。此外，这一机制提供了一个新的模型形态梯度的形成。
Formation and patterning of the vertebrate embryo occur in a head-to-tail sequence. This progressive mode of body formation from the posterior end of the embryo requires a strict temporal coordination of tissue differentiation - a process involving fibroblast growth factor (FGF) signalling. Here we show that transcription of fgf8 messenger RNA is restricted to the growing posterior tip of the embryo. fgf8 mRNA is progressively degraded in the newly formed tissues, resulting in the formation of an mRNA gradient in the posterior part of the embryo. This fgf8 mRNA gradient is translated into a gradient of FGF8 protein, which correlates with graded phosphorylation of the kinase Akt, a downstream effector of FGF signalling. Such a mechanism provides an efficient means to monitor the timing of FGF signalling, coupling the differentiation of embryonic tissues to the posterior elongation of the embryo. In addition, this mechanism provides a novel model for morphogen gradient formation.