P2X7 Receptor Expression in Peripheral Blood Monocytes Is Correlated With Plasma C-Reactive Protein and Cytokine Levels in Patients With Type 2 Diabetes Mellitus: a Preliminary Report
P2X7 Receptor Expression in Peripheral Blood Monocytes Is Correlated With Plasma C-Reactive Protein and Cytokine Levels in Patients With Type 2 Diabetes Mellitus: a Preliminary Report
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2 型糖尿病患者外周血单核细胞中 P2X(7) 受体表达与血浆 C 反应蛋白和细胞因子水平相关：初步报告
DOI：
10.1007/s10753-015-0189-y
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发表时间：
2015-12-01
期刊：
影响因子：
5.1
通讯作者：
Liang, Shangdong
中科院分区：
文献类型：
--
作者：
Wu, Hong;Nie, Yijun;Liang, Shangdong
慢性炎症在2型糖尿病（T2 DM）的发展中起主要作用。C-反应蛋白（CRP）和炎性细胞因子如肿瘤坏死因子-α（TNF-α）和白细胞介素1 β（IL-1 β）直接参与胰岛素抵抗的发生。细胞外ATP水平的增加可以通过P2 X（7）受体放大体内的炎症反应。本研究旨在评估T2 DM患者外周血单核细胞P2 X（7）受体表达与TNF-α、IL-1 β和CRP血浆水平之间的关系。结果显示单核细胞P2 X（7）受体表达增加与高血清CRP、TNF-α和IL-1 β水平相关。TNF-α和IL-1 β水平在健康受试者中最低;在T2 DM患者中，与CRP含量高的受试者相比，CRP水平正常的受试者中这些炎症标志物的含量较低。相反，在CRP水平高的T2 DM患者中，IL-10水平显著降低。与健康个体和CRP水平正常的T2 DM患者相比，CRP水平高的T2 DM患者单核细胞中P2 X（7）受体表达显著增加。提示外周血单核细胞P2 X（7）受体可能参与了T2 DM的病理变化，尤其是影响高CRP患者。
Chronic inflammation plays a major role in development of type 2 diabetes mellitus (T2DM). C-reactive protein (CRP) and inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) are directly involved in the occurrence of insulin resistance. Increased extracellular ATP levels can amplify the inflammatory response in vivo via the P2X(7) receptor. The present study aimed to assess the relationship between P2X(7) receptor expression in human peripheral blood monocytes and plasma levels of TNF-alpha, IL-1 beta, and CRP in T2DM patients. The results showed the association of increased P2X(7) receptor expression of monocytes with high serum CRP, TNF-alpha, and IL-1 beta levels. TNF-alpha and IL-1 beta levels were lowest in healthy subjects; in T2DM patients, these inflammatory markers were less abundant in individuals with normal CRP levels compared to those with high CRP contents. In contrast, IL-10 levels in T2DM patients with high CRP levels were dramatically decreased. P2X(7) receptor expression in monocytes from T2DM patients with high CRP levels was significantly increased in comparison with healthy individuals and T2DM patients with normal CRP levels. These findings indicated that P2X(7) receptor in peripheral blood monocytes may be involved in the pathological changes of T2DM, particularly affecting patients with high CRP levels.