Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker
Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker
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DOI：
10.3390/molecules24081521
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发表时间：
2019-04-02
期刊：
影响因子：
4.6
通讯作者：
Xiong, Yin
中科院分区：
文献类型：
--
作者：
Li, Yehong;Zhang, Yiming;Xiong, Yin
天麻(G.elata)块茎是一种治疗多种疾病的珍贵中草药。建立合理可行的天麻药材质量评价方法是保证其临床安全性和有效性的重要环节。本研究以高效液相色谱指纹图谱分析为基础，结合多组分的单标记定量分析，建立了一种有效的、全面的天麻药材质量评价方法。以天麻素为参比标准，同时测定了天麻素、对羟基苯甲醇、对羟基苯甲醛、天麻素A、天麻素B、天麻素C、天麻素E等7种成分的含量。结果表明，QAMS法与传统的外标法测定结果无显著差异(p>；0.05，RSD<；4.79%)，说明QAMS法是一种可靠、简便的多组分含量测定方法，特别是在缺乏标准品的情况下。总之，该策略有利于简化天麻药材的质量控制流程，为提高药材质量标准提供参考。
Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines.