Mannheimia haemolytica biofilm formation on bovine respiratory epithelial cells
Mannheimia haemolytica biofilm formation on bovine respiratory epithelial cells
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DOI：
10.1016/j.vetmic.2016.11.012
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发表时间：
2016-12-25
影响因子：
3.3
通讯作者：
Czuprynski, Charles J.
中科院分区：
文献类型：
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作者：
Boukahil, Ismail;Czuprynski, Charles J.
溶血曼海姆菌是与牛呼吸道疾病综合征 (BRDC) 相关的最重要的细菌病原体，该疾病给养牛业造成了全球性的经济损失。溶血支原体细胞最初定植于牛上呼吸道的扁桃体隐窝，随后它们可以从那里下降到肺部引起疾病。许多细菌以生物膜的形式存在于宿主体内。我们假设，在共生状态下，溶血支原体在牛体内的定植可能包括生物膜的形成。为了开始评估这种可能性，我们开发了一种体外系统来直接研究牛呼吸道上皮细胞上的生物膜形成。使用固定的原代牛支气管上皮细胞，我们在 37℃ 孵育 48 小时后观察到溶血分枝杆菌生物膜的形成。添加粘蛋白（上呼吸道粘液的主要成分）可减少牛上皮细胞上溶血分枝杆菌生物膜的形成。我们研究了上皮细胞先前病毒感染对随后溶血分枝杆菌生物膜形成的影响，发现影响可以忽略不计。该模型系统的利用将为溶血分枝杆菌生物膜形成在 BRDC 发病机制中的潜在作用提供新的见解。 (C) 2016 Elsevier B.V. 保留所有权利。
Mannheimia haemolytica is the most important bacterial agent associated with the bovine respiratory disease complex (BRDC), which causes worldwide economic losses to the cattle industry. M. haemolytica cells initially colonize the tonsillar crypts in the upper respiratory tract of cattle, from where they can subsequently descend into the lungs to cause disease. Many bacteria exist as biofilms inside their hosts. We hypothesize that M. haemolytica colonization of cattle during its commensal state may include biofilm formation. To begin to assess this possibility, we developed an in vitro system to study biofilm formation directly on bovine respiratory epithelial cells. Using fixed primary bovine bronchial epithelial cells, we observed M. haemolytica biofilm formation after a 48 h incubation period at 37 C. Addition of mucin, the main component of mucus present in the upper respiratory tract, decreased M. haemolytica biofilm formation on bovine epithelial cells. We investigated the effects of prior viral infection of the epithelial cells on subsequent biofilm formation by M. haemolytica and found negligible effects. Utilization of this model system will provide new insights into the potential role of biofilm formation by M. haemolytica in the pathogenesis of BRDC. (C) 2016 Elsevier B.V. All rights reserved.