Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression.
Influence of the pattern size of micropatterned scaffolds on cell morphology, proliferation, migration and F-actin expression.
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DOI：
10.1039/c3bm60237k
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发表时间：
2014-02
影响因子：
6.6
通讯作者：
H. Sunami;Ikuko Yokota;Y. Igarashi
中科院分区：
文献类型：
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作者：
H. Sunami;Ikuko Yokota;Y. Igarashi
为了确定支架的三维形状对细胞功能的影响，在硅衬底上制备了不同尺寸的三维微图案化支架。微结构为长3~20μm的等边三角形孔洞，具有相同的孔隙率(单位面积总孔面积)和深度。这些图案只是在其2D大小方面有所不同。这样的支架以前没有被生成过，因此还没有解决图案大小对细胞功能的影响。将NIH-3T3细胞培养在这些微图案化支架上，观察其形态、增殖率、迁移率和F-肌动蛋白的表达水平。随着支架图案尺寸的减小，细胞变得更圆，F-肌动蛋白表达减少。模式大小与细胞增殖和迁移之间的关系也得到了证实。这些结果表明，三维微图案化支架的图案尺寸影响细胞承受的机械应力水平，从而影响F-肌动蛋白的表达、细胞形态、细胞增殖和细胞迁移。
To determine how the three-dimensional (3D) shape of scaffolds influences cell functions, 3D micropatterned scaffolds of various sizes were fabricated on a silicon substrate. The micropatterns were equilateral triangular pores with 3-20 μm long sides, and all had the same pore ratio (total pore area per unit area) and depth. The patterns only differed in terms of their 2D size. Such scaffolds have not been previously generated, and thus the effects of pattern size on cell functions have not been addressed. NIH-3T3 cells were cultured on these micropatterned scaffolds, and their morphology, proliferation rate, migration rate, and level of F-actin expression were assessed. Cells became more rounded and F-actin expression decreased as the pattern size of the scaffold decreased. Relationships were also demonstrated between pattern size and cell proliferation and migration. These results suggest that the pattern size of 3D micropatterned scaffolds affects the level of mechanical stress that cells experience, and thereby influences F-actin expression, cell morphology, cell proliferation and cell migration.