Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells
Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells
复制标题
DOI：
10.1038/s41598-019-46264-y
复制
发表时间：
2019-07-08
影响因子：
4.6
通讯作者：
Gui, Bao-Song
中科院分区：
文献类型：
--
作者：
Han, Jin;Guo, Da;Gui, Bao-Song
本研究通过观察3种不同分子量的黄芪多糖（APS）对损伤的人肾小管上皮细胞（HK-2）的修复作用，探讨多糖分子量对细胞修复的影响。通过用2.6mM草酸损伤HK-2细胞建立损伤模型，并使用分子量为11.03、4.72和2.61KDa的APS 0、APS 1和APS 2来修复损伤的细胞。经APS修复后，受损的HK-2细胞形态逐渐恢复正常，细胞间连接的破坏得到恢复，细胞内活性氧产生量减少，线粒体数量减少。膜电位升高。修复后细胞周期进程逐渐正常化，溶酶体完整性增强，细胞凋亡率明显下降。3种APS均能促进Keap 1、Nrf 2、SOD 1和CAT的表达。此外，APS修复后炎症标志物MCP-1和IL-6的表达水平降低。我们推测APS通过激活Nrf 2-Keapl信号通路和抑制炎症来发挥其修复功能。在APS中，具有中等Mw的APS 1提供了最强的修复效果。APS可能对肾结石有预防作用。
This study investigated the repair effects of three Astragalus polysaccharides (APSs) with different molecular weights (Mws) on injured human renal proximal tubular epithelial (HK-2) cells to reveal the effect of Mw of polysaccharide on cell repair. A damage model was established by injuring HK-2 cells with 2.6 mM oxalate, and APS0, APS1, and APS2 with Mw of 11.03, 4.72, and 2.61 KDa were used to repair the damaged cells. After repair by APSs, the morphology of damaged HK-2 cells gradually returned to normal, the destruction of intercellular junctions recovered, intracellular reactive oxygen species production amount decreased, and their mitochondria! membrane potential increased. In addition, the cell cycle progression gradually normalized, lysosome integrity increased, and cell apoptotic rates obviously declined in the repaired cells. All three APSs could promote the expression of Keap1, Nrf2, SOD1, and CAT. In addition, the expression levels of inflammation markers containing MCP-1 and IL-6 decreased after APS repair. We deduced that APSs exert their repair function by activating the Nrf2-Keapl signaling pathway and inhibiting inflammation. Among the APSs, APS1 with a moderate Mw provided the strongest repair effect. APSs may have a preventive effect on kidney stones.