Molecular Characterization of Two Highly Homologous Receptor-like Kinase Genes, RLK902 and RKL1, in Arabidopsis thaliana
Molecular Characterization of Two Highly Homologous Receptor-like Kinase Genes, RLK902 and RKL1, in Arabidopsis thaliana
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DOI：
10.1271/bbb.68.1935
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发表时间：
2004-01
期刊：
影响因子：
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通讯作者：
Y. Tarutani;T. Morimoto;A. Sasaki;M. Yasuda;H. Nakashita;S. Yoshida;I. Yamaguchi;Yoshihito Suzuki
中科院分区：
文献类型：
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作者：
Y. Tarutani;T. Morimoto;A. Sasaki;M. Yasuda;H. Nakashita;S. Yoshida;I. Yamaguchi;Yoshihito Suzuki
受体样激酶（Receptor-like kinases，RLK）是一类重要的信号感知分子。我们在拟南芥中鉴定了两个高度同源的RLK基因，RLK 902和RKL 1。RLK 902和RKL 1在其整个区域上显示75%的氨基酸序列同一性。在RLK 902 pro：：GUS转基因株系中，GUS活性在根尖、侧根原基、托叶和花器官分离区表现较强，而RKL 1启动子活性在气孔细胞、莲座叶的毛状体和气孔器细胞以及花器官分离区表现较强。在正常生长条件下，rlk 902突变株系、rkl 1突变株系和rlk 902/rkl 1双敲除突变株系均未表现出任何显著的表型。这些结果表明，RLK 902和RKL 1可能介导的信号转导途径中，至少有一个其他的互补信号通路，这两个RLK可能存在。
Receptor-like kinases (RLKs) constitute a large family of signal perception molecules. We characterized two highly homologous RLK genes, RLK902 and RKL1, in Arabidopsis. RLK902 and RKL1 showed a 75% amino acid sequence identity over their entire regions. In the RLK902 pro::GUS transgenic lines, GUS activity was strong in the root tips, lateral root primordia, stipules, and floral organ abscission zones, while the RKL1 promoter activity was dominant in the stomata cells, hydathodes and trichomes of young rosette leaves, and floral organ abscission zones. Neither the rlk902 mutant line, rkl1 mutant line nor rlk902/rkl1 double-knockout mutant line showed any significant phenotypes under normal growth conditions. These results suggest that RLK902 and RKL1 might mediate the signal transduction pathway in which at least one other complementary signaling pathway to these two RLKs might exist.