IN-VITRO EFFECTS OF RECOMBINANT HUMAN INTERFERON-GAMMA ON HUMAN MESOTHELIOMA CELL-LINES
IN-VITRO EFFECTS OF RECOMBINANT HUMAN INTERFERON-GAMMA ON HUMAN MESOTHELIOMA CELL-LINES
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DOI：
10.1002/ijc.2910550331
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发表时间：
1993-09-30
影响因子：
6.4
通讯作者：
JAURAND, MC
中科院分区：
文献类型：
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作者：
ZENG, L;BUARD, A;JAURAND, MC
恶性间皮瘤是一种起源于浆液性表面的肿瘤，通常与石棉接触有关。恶性间皮瘤对各种形式的治疗具有抵抗力。放疗、手术或化疗只能略微改善预后。干扰素-γ在I期恶性间皮瘤患者中产生完全或部分反应。干扰素-γ对恶性间皮瘤细胞的体外生物学作用尚不清楚。本研究用四种剂量的重组人干扰素-γ作用于32株人间皮瘤细胞系(HMCL)，用比色法(四甲基偶氮唑盐比色法)检测细胞生长情况。在所检测的32个HMCL中，11个表现出明显的细胞生长抑制，16个对r-Hu干扰素-γ不敏感，S被轻微抑制。R-Hu干扰素-γ对敏感细胞株有较强的抑制作用。我们的结果表明，HMCL对r-Hu干扰素-γ的反应范围很大，其中一些反应可以与在人体内获得的反应进行比较。(C)1993年Wiley-Liss，Inc.
Malignant mesothelioma is a tumor arising from serous surfaces and often related to asbestos exposure. Malignant mesothelioma is resistant to various forms of therapy. Radiotherapy, surgery or chemotherapy only slightly improve prognosis. IFN-gamma produces complete or partial responses in stage-I patients with malignant mesothelioma. The in vitro biological effect of IFN-gamma on malignant mesothelioma cells remains poorly elucidated. In the present study, 32 well-characterized human mesothelioma cell lines (HMCL) were treated with r-hu IFN-gamma at 4 doses and cell growth was determined by a colorimetric method (MTT assay). Among the 32 HMCLs tested, 11 exhibited significant cell-growth inhibition; 16 were insensitive to r-hu IFN-gamma, and S were slightly inhibited. The sensitive cell lines were strongly inhibited by r-hu IFN-gamma. Our results show that HMCL exhibit a large range of responses to r-hu IFN-gamma, some of which can be compared with those obtained in vivo in humans. (C) 1993 Wiley-Liss, Inc.