Endurance exercise training reduces cardiac sodium/calcium exchanger expression in animals susceptible to ventricular fibrillation.
Endurance exercise training reduces cardiac sodium/calcium exchanger expression in animals susceptible to ventricular fibrillation.
复制标题
DOI：
10.3389/fphys.2011.00003
复制
发表时间：
2011
影响因子：
4
通讯作者：
Billman GE
中科院分区：
文献类型：
--
作者：
Kukielka M;Holycross BJ;Billman GE
目的：钠/钙交换体活性（NCX 1，心肌细胞胞浆钙的重要调节因子）增加可能引起心律失常。运动训练可以降低心力衰竭动物的NCX 1表达，改善细胞溶质钙调节，从而降低心室颤动（VF）的风险。研究方法：为了验证这一假设，在心肌梗死愈合的犬中，在运动的最后一分钟进行了2分钟的冠状动脉闭塞; 23只有VF（S，敏感），13只没有（R，抵抗）。动物被随机分配到10周的运动训练（逐步增加跑步机跑步; S n = 9; R n = 8）或10周的久坐（S n = 14; R n = 5）组。在10周结束时，运动+缺血试验在久坐但未训练的易感犬中引起VF。在随后的一天，收获心脏组织，并通过Western印迹测定NCX 1蛋白表达。结果：在安静组，易感犬的NCX 1表达显著高于耐药犬（ANOVA，P < 0.05）。相比之下，NCX 1水平在运动训练的抵抗和易感动物中相似。结论：这些数据表明，运动训练可以使VF易感犬恢复更正常的NCX 1水平，改善Cyr钙调节，从而降低心肌梗死后猝死的风险。
Aim: Increased sodium/calcium exchanger activity (NCX1, an important regulator of cardiomyocyte cystolic calcium) may provoke arrhythmias. Exercise training can decrease NCX1 expression in animals with heart failure improving cytosolic calcium regulation, and could thereby reduce the risk for ventricular fibrillation (VF). Methods: To test this hypothesis, a 2-min coronary occlusion was made during the last minute of exercise in dogs with healed myocardial infarctions; 23 had VF (S, susceptible) and 13 did not (R, resistant). The animals were randomly assigned to either 10-week exercise training (progressively increasing treadmill running; S n = 9; R n = 8) or 10-week sedentary (S n = 14; R n = 5) groups. At the end of the 10-week period, the exercise + ischemia test provoked VF in sedentary but not trained susceptible dogs. On a subsequent day, cardiac tissue was harvested and NCX1 protein expression was determined by Western blot. Results: In the sedentary group, NCX1 expression was significantly (ANOVA, P < 0.05) higher in susceptible compared to resistant dogs. In contrast, NCX1 levels were similar in the exercise trained resistant and susceptible animals. Conclusion: These data suggest that exercise training can restore a more normal NCX1 level in dogs susceptible to VF, improving cystolic calcium regulation and could thereby reduce the risk for sudden death following myocardial infarction.
登录
查看更多内容
影响因子：
20.1
作者：
Armoundas, AA;Hobai, IA;O'Rourke, B
通讯作者：
O'Rourke, B
影响因子：
2.8
作者：
HERTZEANU, HL;SHEMESH, J;KELLERMANN, JJ
通讯作者：
KELLERMANN, JJ
影响因子：
3.3
作者：
Houle, MS;Altschuld, RA;Billman, GE
通讯作者：
Billman, GE
影响因子：
37.8
作者：
Despa, Sanda;Tucker, Amy L.;Bers, Donald M.
通讯作者：
Bers, Donald M.
DOI：
10.1152/ajpheart.00763.2007
发表时间：
2007-11-01
影响因子：
4.8
作者：
Holycross, Bethany J.;Kukielka, Monica;Billman, George E.
通讯作者：
Billman, George E.