Spy1, a histidine-containing phosphotransfer signaling protein, regulates the fission yeast cell cycle through the Mcs4 response regulator
Spy1, a histidine-containing phosphotransfer signaling protein, regulates the fission yeast cell cycle through the Mcs4 response regulator
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DOI：
10.1128/jb.182.17.4868-4874.2000
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发表时间：
2000-09-01
影响因子：
3.2
通讯作者：
Mizuno, T
中科院分区：
文献类型：
--
作者：
Aoyama, K;Mitsubayashi, Y;Mizuno, T
常见的组氨酸到天冬氨酸 (His-to-Asp) 磷酸中继信号系统涉及三种类型的信号传导组件：传感器 His 激酶、反应调节器和含组氨酸的磷酸转移 (HPt) 蛋白。最近在裂殖酵母裂殖酵母中发现了两种反应调节因子 Mcs4 和 Prr1，并且表明它们参与了应激反应中涉及的信号转导。此外，Mcs4 似乎参与有丝分裂细胞周期控制。然而，HPt 磷酸递质和 His 激酶均未在粟酒裂殖酵母中得到表征。在这项研究中，我们鉴定了一个编码 HPt 磷酸递质的基因，命名为 Spy1（粟酒裂殖酵母 YPD1 样蛋白）。 SPY1(+)基因显示出能够补充酿酒酵母YPD1基因的突变损伤，该基因参与渗透传感信号转导。酵母双杂交分析结果表明Spy1与Mcs4存在相互作用。为了深入了解 Spy1 的功能，进行了一系列遗传分析。结果证明 Spy1 与 Mcs4 一起在 G(2)/M 细胞周期进程的调节中发挥作用。 Spy1 缺陷的细胞似乎提前进入 M 期。在所提出的模型中，Spy1 以负向方式调节 Mcs4，可能是通过直接的 His-to-Asp 磷酸中继，在 Sty1 丝裂原激活蛋白激酶级联的上游运行。
Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been identified recently, and it was shown that they are involved in the signal transduction implicated in stress responses. Furthermore, Mcs4 appears to be involved in mitotic cell-cycle control. However, neither the HPt phosphotransmitter nor His kinase has been characterized in S. pombe. In this study, we identified a gene encoding an HPt phosphotransmitter, named Spy1 (S. pombe YPD1-like protein). The spy1(+) gene showed an ability to complement a mutational lesion of the Saccharomyces cerevisiae YPD1 gene, which Is involved in an osmosensing signal transduction. The result from yeast two-hybrid analysis indicated that Spy1 interacts with Mcs4. To gain insight into the function of Spy1, a series of genetic analyses were conducted. The results provided evidence that Spy1, together with Mcs4 plays a role in regulation of the G(2)/M cell cycle progression. Spy1-deficient cells appear to be precocious in the entry to M phase. In the proposed model, Spy1 modulates Mcs4 in a negative manner, presumably through a direct His-to-Asp phosphorelay, operating upstream of the Sty1 mitogen-activated protein kinase cascade.