Protamine sulfate inhibits mitogenic activities of the extracellular matrix and fibroblast growth factor, but potentiates that of epidermal growth factor.
Protamine sulfate inhibits mitogenic activities of the extracellular matrix and fibroblast growth factor, but potentiates that of epidermal growth factor.
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硫酸鱼精蛋白抑制细胞外基质和成纤维细胞生长因子的有丝分裂活性，但增强表皮生长因子的有丝分裂活性。
DOI：
10.1002/jcp.1041320213
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发表时间：
1987
影响因子：
5.6
通讯作者：
Gospodarowicz,D
中科院分区：
文献类型：
--
作者：
Neufeld,G;Gospodarowicz,D
硫酸鱼精蛋白是体内血管生成的抑制剂，可显著抑制酸性或碱性成纤维细胞生长因子等血管生成因子刺激BHK-21细胞和血管内皮细胞体外增殖的能力。这种抑制是可逆的，即使在长期暴露于鱼精蛋白硫酸盐的情况下，细胞仍能存活。硫酸鱼精蛋白通过阻止这两种生长因子与它们共同的细胞表面受体结合来抑制它们的有丝分裂作用。它还抑制牛角膜内皮细胞产生的细胞外基质的有丝分裂活性。以前的研究表明，这种底物可以取代许多细胞类型对成纤维细胞生长因子的需求。相反，硫酸鱼精蛋白增强了表皮生长因子(EGF)的有丝分裂活性。这表明，硫酸鱼精蛋白也作用于与成纤维细胞生长因子受体无关的细胞部位。
Protamine sulfate, an inhibitor of angiogenesis in vivo, markedly inhibits the ability of angiogenic factors such as acidic or basic fibroblast growth factor (aFGF, bFGF) to stimulate the proliferation in vitro of either BHK‐21 cells or vascular endothelial cells. The inhibition is reversible, and cells remain viable even after prolonged exposure to protamine sulfate. Protamine sulfate inhibits the mitogenic effects of both growth factors by preventing them from binding to their common cell surface receptors. It also inhibits the mitogenic activity of the extracellular matrix produced by bovine corneal endothelial cells. This substrate has been shown in previous studies to replace the requirement for FGF of many cell types. In contrast, protamine sulfate potentiates the mitogenic activity of epidermal growth factor (EGF). This indicates that protamine sulfate also acts at cellular sites which are not associated with FGF receptors.