Ca2+ Signaling and Homeostasis in Mammalian Oocytes and Eggs.
Ca2+ Signaling and Homeostasis in Mammalian Oocytes and Eggs.
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DOI：
10.1101/cshperspect.a035162
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发表时间：
2019-08
影响因子：
7.2
通讯作者：
T. Wakai;Aujan Mehregan;R. Fissore
中科院分区：
文献类型：
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作者：
T. Wakai;Aujan Mehregan;R. Fissore
细胞内钙离子浓度([Ca~(2+)]i)的变化代表了一种重要的信号机制，使细胞之间以及细胞与环境之间的交流成为可能。细胞已经开发出一套复杂的分子，“钙工具箱”，以适应[钙]i的变化，以特定的细胞功能。哺乳动物卵母细胞和卵子也不例外，事实上，所有物种胚胎发育的启动完全依赖于不同的[Ca~(2+)]i反应。在这里，我们回顾了存在于哺乳动物卵母细胞和卵子中的钙工具包的组成，以及允许这些细胞在内质网中积累和释放钙并维持基础和稳定的细胞质浓度的调节机制。我们还讨论了在卵母细胞和卵子中发现的钙离子内流通道的电生理和遗传学研究，并分析了支持精子特异性磷脂酶C亚型在哺乳动物受精过程中触发钙振荡的证据，包括它在生育方面的意义。
Changes in the intracellular concentration of calcium ([Ca2+]i) represent a vital signaling mechanism enabling communication between and among cells as well as with the environment. Cells have developed a sophisticated set of molecules, "the Ca2+ toolkit," to adapt [Ca2+]i changes to specific cellular functions. Mammalian oocytes and eggs, the subject of this review, are not an exception, and in fact the initiation of embryo devolvement in all species is entirely dependent on distinct [Ca2+]i responses. Here, we review the components of the Ca2+ toolkit present in mammalian oocytes and eggs, the regulatory mechanisms that allow these cells to accumulate Ca2+ in the endoplasmic reticulum, release it, and maintain basal and stable cytoplasmic concentrations. We also discuss electrophysiological and genetic studies that have uncovered Ca2+ influx channels in oocytes and eggs, and we analyze evidence supporting the role of a sperm-specific phospholipase C isoform as the trigger of Ca2+ oscillations during mammalian fertilization including its implication in fertility.