Alterations of inflammatory cytokines in super-acute stroke patients and the potential pathogenesis
Alterations of inflammatory cytokines in super-acute stroke patients and the potential pathogenesis
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DOI：
10.1016/j.jocn.2022.02.034
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发表时间：
2022-02-28
影响因子：
2
通讯作者：
Zhao, Haiping
中科院分区：
文献类型：
--
作者：
Li, Fangfang;Ma, Qingfeng;Zhao, Haiping
背景资料：充分了解脑卒中后的全身炎症反应将使针对炎症的治疗策略更加可行。在这里，我们的目的是确定全球性的变化，循环细胞因子在超急性缺血性stroke（AIS），方法：一个广泛的面板65细胞因子测定28例AIS患者（n = 28），脑出血患者（n = 28）和健康对照组（n = 18）在6小时内中风发作后的血浆中。采用受试者工作特征曲线（ROC）和斯皮尔曼等级相关分析候选细胞因子的诊断效能及其与淋巴细胞和中性粒细胞数量的关系。血浆IL-1 β、IL-2、IL-2 R、IL-5、IL-10、CD 40 L、HGF、MIP-3 α、MMP-1表达水平明显上调，而与健康对照相比，AIS患者中IL-16下调。其中，IL-2 R、IL-10、IL-16、MIP-3 α和MMP-1在AIS患者中特异性改变，而IL-1 β、IL-2、IL-5、CD 40 L和HGF在AIS和出血性卒中患者中同时升高。有趣的是，IL-6和TNF-β被发现是65种细胞因子中区分出血和缺血的关键因子。AIS患者IL-1 β、IL-16、CD 40 L、HGF与中性粒细胞数显著相关，IL-1 β、IL-16与中性粒细胞数显著相关。结论：AIS和出血除了某些共同的病理过程外，还有其独特的发病机制，将这一认识转化为进一步的研究，可能为AIS的治疗提供新的策略。
Background: Sufficient understanding of the systemic inflammatory response after stroke will make the therapeutic strategy targeting inflammation more feasible. Here, we aimed to identify the globally alterations of circulating cytokines in super-acute ischemic stroke (AIS).Methods: A broad panel of 65 cytokines was measured in the plasma of twenty-eight AIS patients within 6 h after stroke onset (n = 28), cerebral hemorrhagic patients (n = 28) and healthy controls (n = 18). The diagnostic power of the candidate cytokines and their relationship with the number of lymphocytes and neutrophils were analyzed by receiver operating characteristic (ROC) and spearman rank correlation respectively.Results: The expression level of plasma IL-1beta, IL-2, IL-2R, IL-5, IL-10, CD40L, HGF, MIP-3alpha and MMP-1 were obviously up-regulated, while IL-16 was down-regulated in AIS patients compared to healthy controls. Among them, IL-2R, IL-10, IL-16, MIP-3alpha, and MMP-1 were specially altered in AIS patients, while IL-1beta, IL-2, IL-5, CD40L and HGF were elevated simultaneously in AIS and hemorrhagic stroke patients. Interestingly, IL-6 and TNF-beta were found to be key facytors among the 65 cytokines to distinguish hemorrhage from ischemia. Furthermore, IL-1beta, IL-16, CD40L and HGF were obviously correlated with the number of lym-phocytes, and IL-1beta and IL-16 were significantly associated with the number of neutrophils in AIS patients. These results suggest that lymphocytes and neutrophils associated inflammation may play a pivotal role in AIS.Conclusions: Importantly, except for some mutual pathological processes, AIS and hemorrhage had their own distinctive pathogenesis, and transformation of this knowledge to further research may provide novel treatment strategy for AIS.