Amplification of the TCL1 flanking region at 14q32.1 with no TCL1 gene transcription in a patient with peripheral T cell lymphoma
Amplification of the TCL1 flanking region at 14q32.1 with no TCL1 gene transcription in a patient with peripheral T cell lymphoma
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外周 T 细胞淋巴瘤患者 14q32.1 处 TCL1 侧翼区域的扩增，无 TCL1 基因转录
DOI：
10.1038/sj.leu.2401023
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发表时间：
1998
期刊：
影响因子：
11.4
通讯作者：
Y. Kaneko
中科院分区：
文献类型：
--
作者：
A. Sakashita;H. Kobayashi;N. Satake;N. Maseki;M. Sakurai;T. Izumo;M. Isobe;Y. Kaneko
除成人 T 细胞白血病/淋巴瘤 (ATL/L) 外，外周 T 细胞淋巴瘤 (PTL) 的细胞遗传学和分子遗传学特征尚未明确定义。涉及染色体带 14q32 的易位和倒位是 PTL 和 ATL/L 中极为常见的异常。我们使用 TCL1 基因侧翼的两个粘粒探针通过两色荧光原位杂交 (FISH) 研究了 TCL1（一种最近分离的位于 14q32.1 的基因）在 20 名 PTL 患者肿瘤组织中的参与情况，其中 3 名患者有 14q32 易位。两个粘粒信号在它们中均未分离，但在没有 14q32 易位的一种肿瘤中数量大大增加，表明 TCL1 基因组区域在该肿瘤中被扩增。然而，逆转录聚合酶链反应（RT-PCR）未能检测到肿瘤中的 TCL1 转录本。这些发现提示TCL1以外的癌基因可能位于14q32.1，其扩增可能参与PTL的肿瘤过程。
Cytogenetic and molecular-genetic characteristics in peripheral T cell lymphoma (PTL) have not been well defined, except for those in adult T cell leukemia/lymphoma (ATL/L). Translocations and inversions involving a chromosome band 14q32 were extremely common abnormalities reported in PTL and ATL/L. We studied the involvement of TCL1, a recently isolated gene located in 14q32.1, in tumor tissues from 20 patients with PTL including three with 14q32 translocations by two color fluorescent in situ hybridization (FISH) using two cosmid probes flanking the TCL1 gene. The two cosmid signals were separated in none of them, but much increased in number in one tumor without 14q32 translocation, indicating that the TCL1 genomic region was amplified in this tumor. Reverse transcription-polymerase chain reaction (RT-PCR), however, failed to detect the TCL1 transcript in the tumor. These findings suggest that an oncogene other than TCL1 may be located in 14q32.1, and its amplification may be involved in the neoplastic process of PTL.