Fluorination Enhances the Osteogenic Capacity of Porcine Hydroxyapatite
Fluorination Enhances the Osteogenic Capacity of Porcine Hydroxyapatite
复制标题
氟化增强猪羟基磷灰石的成骨能力
DOI：
10.1089/ten.tea.2017.0381
复制
发表时间：
2018-03-01
影响因子：
4.1
通讯作者：
Chen, Zhuofan
中科院分区：
文献类型：
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作者：
Liu, Runheng;Qiao, Wei;Chen, Zhuofan
在先前的研究中，我们成功地制备了氟化猪羟基磷灰石（FPHA）通过浸泡在0.25 M氟化钠（NaF）的水溶液中的热处理，并得到的FPHA显示出更好的物理化学和生物学性能比PHA。本研究的目的是进一步研究氟掺入如何影响PHA的生物相容性和成骨能力。用电感耦合等离子体发射光谱法测定PHA和FPHA提取物中Ca、P、F和Mg离子的浓度。用PHA和FPHA提取物处理大鼠骨髓基质细胞（rBMSCs），通过细胞计数试剂盒-8法、碱性磷酸酶法和真实的时间定量聚合酶链反应（RT-PCR）检测其对细胞增殖和成骨分化的影响。将PHA和FPHA植入大鼠颅骨缺损（直径5 mm，n= 14）和皮下囊袋（n= 6）中12周后，通过显微CT和组织学分析，观察其生物相容性和成骨能力。FPHA提取物释放出较高浓度的F和Mg离子，能更好地促进rBMSCs体外向成骨细胞分化。生物相容性评价结果证实，PHA和FPHA颗粒周围的宿主反应和慢性炎症细胞浸润程度相似。显微CT和组织学分析显示，与PHA治疗的缺损大鼠相比，FPHA治疗的缺损大鼠有更新的矿化骨形成。体外和体内实验结果一致表明，氟掺入有效地增强了PHA的成骨能力。
In a previous study, we successfully prepared fluorinated porcine hydroxyapatite (FPHA) by immersing porcine hydroxyapatite (PHA) in an aqueous solution of 0.25 M sodium fluoride (NaF) under thermal treatment, and the resulting FPHA showed better physicochemical and biological properties than PHA. The purpose of this study was to further investigate how fluorine incorporation influenced the biocompatibility and osteogenic capacity of PHA. The concentrations of Ca, P, F, and Mg ions in PHA and FPHA extracts were detected by inductively coupled plasma optical emission spectrometry. Rat bone marrow stromal cells (rBMSCs) were treated with PHA and FPHA extracts, and the effects of these extracts on cell proliferation and osteoblastic differentiation were evaluatedviaCell Counting Kit-8 assay, alkaline phosphatase assay, and real time-quantitative polymerase chain reaction. For thein vivoassessment, PHA and FPHA were implanted into subcutaneous pockets (n= 6) and rat calvarial defects (diameter = 5 mm,n= 14) for 12 weeks to determine their biocompatibility and osteogenic capacity by using micro-computed tomography (CT) and histological analysis. FPHA extracts, which release higher concentrations of F and Mg ions, better promoted the osteoblastic differentiation of rBMSCsin vitro. The result of biocompatibility evaluation confirmed that the host response and chronic inflammation cells infiltration degree around PHA and FPHA granules were similar. Micro-CT and histological analysis showed newer mineralized bone formation in rats with FPHA-treated defects than in rats with PHA-treated defects. The results ofin vitroandin vivotests consistently indicate that fluorine incorporation effectively enhanced the osteogenic capacity of PHA.