Characterization and expression of two amphioxus DDAH genes originating from an amphioxus-specific gene duplication
Characterization and expression of two amphioxus DDAH genes originating from an amphioxus-specific gene duplication
复制标题
源自文昌鱼特异性基因复制的两个文昌鱼 DDAH 基因的表征和表达。
DOI：
10.1016/j.gene.2007.11.021
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发表时间：
2008-02-01
期刊：
影响因子：
3.5
通讯作者：
Zhang, Hongwei
中科院分区：
文献类型：
--
作者：
Chen, Dongyan;Lin, Yushuang;Zhang, Hongwei
对文昌鱼中的两个二甲基精氨酸二甲氨基水解酶（DDAH）同源基因进行了鉴定和克隆。系统发育分析表明，文昌鱼中的两个DDAH起源于头索动物特有的独立重复。对AmphiDDAHa和AmphiDDAHb基因组结构的分析表明，它们与脊椎动物和土著动物的DDAH基因组结构相似，这与动物分类一致。为了探讨AmphiDDAHa和AmphiDDAHb与文昌鱼一氧化氮合酶（NOS）同源物AmphiNOS的功能关系，我们研究了AmphiNOS、AmphiDDAHa和AmphiDDAHb在文昌鱼胚胎和成体组织中的表达模式。结果表明，这三个基因在胚胎发育过程中具有不同的时空表达模式。AmphiDDAHa转录本广泛存在于分化中的外胚层、原肠胚/早期神经胚的中内胚层、神经胚期发育和新形成的神经管、体节、脊索、消化道和表皮以及早期幼虫分化中的咽和尾芽中。而AmphiDDAHb在原肠/早期神经胚分化的非神经外胚层中表达，然后广泛分布于发育形成的神经管、脊索、体节和消化道。AmphiNOS在神经胚早期至72 h幼虫期表达较弱，但广泛表达。在成体中，AmphiDDAHa和AmphiNOS在多种成体组织和细胞中具有相似的表达模式，如神经索、肠、中肠憩室、轮器、鳃、血管、内柱、卵母细胞和巨噬细胞。AmphiDDAHb在上述组织中均未表达。结果提示AmphiDDAHa和AmphiDDAHb可能是文昌鱼中功能不同的两个同源基因。AmphiDDAHa可能在NO合成和免疫防御的调节中起保守作用，而AmphiDDAHb可能主要在原肠胚形成和早期神经胚形成过程中对胚胎非神经外胚层细胞的细胞运动或分化起作用。(C)2007 Elsevier B. V.保留所有权利。
Two dimethylarginine dimethylaminohydrolase (DDAH) homologous genes in amphioxus are identified and cloned. The phylogenetic analysis indicates two DDAHs in amphioxus originated by independent duplication specific in cephalochordate lineage. Analysis of AmphiDDAHa and AmphiDDAHb genomic structure shows their comparability with the DDAH in vertebrate and aboriginality which is consistent with animal classification. To explore the function relationship of AmphiDDAHa and AmphiDDAHb with AmphiNOS, the nitric oxide synthase homologue in amphioxus, we investigate the three genes expression patterns in embryos and adult tissues. The results indicate that these three genes possess different spatial and temporal expression patterns during embryogenesis. AmphiDDAHa transcripts are detected extensively in the differentiating ectoderm, mesendoderm in gastrula/early neurula, the developing and newly formed neural tube, somites, notochord, alimentary canal and epidermis at neurula stage, as well as in the differentiating pharynx and tailbud at early larval stage. While AmphiDDAHb is expressed ill the differentiating non-neural ectoderm at the gastrla/early neurula stage, then locates abroad in the developing and formed neural tube, notochord, somites and alimentary canal. AmphiNOS is expressed weakly but widely from early neurula stage to at least 72-h larva stage. In adult, AmphiDDAHa and AmphiNOS share similar expression patterns in diverse adult tissues and cells such as the neural cord, gut, midgut diverticulum, wheel organ, gill, blood vessels, endostyle, oocytes and macrophages. But no expression of AmphiDDAHb is detected in tissues mentioned above. The results suggest that AmphiDDAHa and AmphiDDAHb should be two homologous genes with different functions in amphioxus. AmphiDDAHa may play a conserved role in the regulation of NO synthesis and immune defense, whereas AmphiDDAHb may mainly play the roles in the cell movement or differentiation of embryonic non-neural ectoderm cells during gastrulation and early neurulation. (C) 2007 Elsevier B.V. All rights reserved.