MicroRNA-126 expression is decreased in cultured primary chicken hepatocytes and targets the sprouty-related EVH1 domain containing 1 mRNA
MicroRNA-126 expression is decreased in cultured primary chicken hepatocytes and targets the sprouty-related EVH1 domain containing 1 mRNA
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MicroRNA-126 表达在培养的原代鸡肝细胞中降低，并靶向包含 1 个 mRNA 的芽相关 EVH1 结构域
DOI：
10.3382/ps.2012-02919
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发表时间：
2013-07-01
期刊：
影响因子：
4.4
通讯作者：
Gu, Zhi-Liang
中科院分区：
文献类型：
--
作者：
Wang, Xing-Guo;Shao, Fang;Gu, Zhi-Liang
microRNA-126（miR-126）是在高度血管化的组织中表达的miRNA，并且被认为通过抑制芽生相关EVH 1结构域1（Spread 1）而在血管生成中发挥作用。本研究确定了鸡miR-126（gga-miR-126）的表达模式，并预测和验证了其靶基因。定量逆转录（qRT）PCR分析表明，miR-126在鸡的多种组织中均有表达，其中以肺组织表达量最高。在肝脏中，miR-126的表达水平从0到7周龄增加。原代鸡肝细胞中miR-126的表达随培养时间的延长而降低。在鸡Spread 1的3' UTR（非翻译区）中预测了miR-126结合位点。双荧光素酶报告基因检测表明，miR-126可以与预测的位点结合，从而抑制Spread 1的表达。这些数据验证了Spread 1作为鸡miR-126的靶基因。这些结果将有助于进一步了解miR-126和Spread 1在鸡中的功能和调控。
The microRNA-126 (miR-126) is a miRNA expressed in highly vascularized tissues, and it is believed to play a role in angiogenesis by repressing sprouty-related EVH1 domain containing 1 (Spred1). In the current study, we determined the expression pattern of chicken miR-126 (gga-miR-126) and predicted and validated its target genes. The quantitative reverse-transcription (qRT) PCR analysis showed that miR-126 was expressed in various chicken tissues with the highest level in lung. In liver, the expression level of miR-126 increased from 0 to 7 wk of age. The expression of miR-126 in primary chicken hepatocytes decreased with culturing. A miR-126 binding site was predicted in the 3' UTR (untranslated region) of chicken Spred1. Dual-luciferase reporter assays indicated that miR-126 could bind to the predicted site to repress the expression of Spred1. These data validate Spred1 as a target gene of chicken miR-126. These results will help further understand the function and regulation of miR-126 and Spred1 in chickens.