Pregnancy increases ovine uterine artery endothelial cyclooxygenase-1 expression
Pregnancy increases ovine uterine artery endothelial cyclooxygenase-1 expression
复制标题
DOI：
10.1210/en.139.2.765
复制
发表时间：
1998-02-01
期刊：
影响因子：
4.8
通讯作者：
Bird, IM
中科院分区：
文献类型：
--
作者：
Janowiak, MA;Magness, RR;Bird, IM
在正常妊娠期间，特别是在妊娠晚期，绵羊子宫动脉的子宫血流量和前列环素的产生都急剧增加。我们试图确定这是否部分归因于子宫动脉内皮中环氧合酶（COX）表达的增加。在本研究中，我们比较了未妊娠和妊娠晚期（妊娠110-142天）母羊子宫动脉内皮中COX的表达。COX-2在子宫动脉内皮和血管平滑肌（VSM）中未见表达。COX-1在子宫动脉中明显表达。COX-1的免疫组化定位为内皮细胞> VSM，两种细胞类型在妊娠晚期均表现出COX-1的升高。胶原酶分散内皮细胞中COX-1蛋白和信使RNA （mRNA）水平均显著升高，在妊娠晚期子宫动脉内皮细胞中COX-1蛋白水平（346.4 +/- 28%,P < 0.0005）和mRNA水平（51.04 +/- 7.98倍，P < 0.001）均显著升高。我们得出结论，妊娠诱导子宫动脉产生前列环素的增加主要是由于COX-1 mRNA和相关蛋白的表达急剧增加，主要发生在子宫动脉内皮中，在较小程度上发生在VSM中。
During normal pregnancy, and especially in the third trimester, both uterine blood flow and prostacyclin production by ovine uterine arteries are dramatically increased. We sought to determine if this is due, in part, to an increase in cyclooxygenase (COX) expression in the uterine artery endothelium. In this study we compared COX expression in uterine artery endothelium from nonpregnant and third-trimester pregnant (110-142 days' gestation) ewes. COX-2 expression was not detectable by Western blotting in uterine artery endothelium or vascular smooth muscle (VSM). In contrast, COX-1 expression was clearly observed in uterine artery. Immunohistochemical localization of COX-1 was endothelium > VSM, with both cell types showing an increase in COX-1 during the third trimester of pregnancy. COX-1 protein and messenger RNA (mRNA) levels were also detectable in collagenase dispersed endothelial cells, with expression of COX-1 in uterine artery endothelial cells dramatically increased during the third trimester of pregnancy at both the level of protein (346.4 +/- 28% of nonpregnant controls, P < 0.0005) and mRNA (51.04 +/- 7.98-fold of nonpregant controls, P < 0.001). We conclude that the pregnancy induced increases in prostacyclin production by uterine arteries is largely due to a dramatic increase in expression of COX-1 mRNA and associated protein predominantly occurring in the uterine artery endothelium and, to a lesser extent, in the VSM.