Studies of chemotactic factor-induced polarity in human neutrophils. Lipid mobility, receptor distribution and the time-sequence of polarization.
Studies of chemotactic factor-induced polarity in human neutrophils. Lipid mobility, receptor distribution and the time-sequence of polarization.
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发表时间：
1991-11
影响因子：
4
通讯作者：
D. McKay;J. Kusel;P. Wilkinson
中科院分区：
文献类型：
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作者：
D. McKay;J. Kusel;P. Wilkinson
用激光显微镜研究了人血中性粒细胞暴露于化学引诱物时前后极膜组成的差异，并测定了引诱物浓度和时间对中性粒细胞粘附能力的影响。调查结果如下。(1)荧光素标记的趋化六肽fLeu-Norleu-Phe-Norleu-Tyr-Lys在极化细胞上不对称分布，在细胞的前极具有最高浓度。(2)膜脂行为的差异进行了研究，荧光恢复后光漂白（FRAP）使用探针5-N-（十八烷酰基）-氨基荧光素。恢复曲线表明，移动的脂质探针在细胞前极的比例高于后极。然而，两极之间的恢复率没有差异。(3)使用暴露于不同各向同性浓度的fMet-Leu-Phe（fMLP）的悬浮液中的中性粒细胞进行极化的时间过程的研究。在低浓度（小于或等于10（-9）M）下，细胞在暴露于引诱剂后立即极化。在高浓度（10（-7）M）下，它们呈现多极形态，极化非常差，表明配体结合太快，细胞无法形成主导极。在最佳浓度10（-8）M fMLP下，细胞在30秒后呈现不规则、皱褶的形态，但在接下来的30分钟内显示极化形式的比例增加。（250字处删节）
Differences in membrane composition between the anterior and posterior poles of human blood neutrophils on exposure to chemoattractant were studied using a laser microscope, and the effects of attractant concentration and time on the ability of neutrophils to polarize were determined. The findings were as follows. (1) The fluorescein-labelled chemotactic hexapeptide fLeu-Norleu-Phe-Norleu-Tyr-Lys was asymmetrically distributed on polarized cells with the highest concentration at the anterior pole of the cell. (2) Differences in membrane lipid behaviour were studied by fluorescence recovery after photobleaching (FRAP) using the probe 5-N-(octadecanoyl)-aminofluorescein. Recovery curves suggested that the proportion of mobile lipid probe at the anterior pole of the cell was higher than at the posterior. However, no difference was found in the rate of recovery between the two poles. (3) Studies of the time-course of polarization were undertaken using neutrophils in suspension exposed to different isotropic concentrations of fMet-Leu-Phe (fMLP). At low concentrations (less than or equal to 10(-9) M), the cells polarized immediately on exposure to the attractant. At a high concentration (10(-7) M) they assumed multipolar morphologies and polarized very poorly, suggesting that ligand binding was too rapid for the cells to form a dominant pole. At the optimal concentration, 10(-8) M fMLP, the cells assumed irregular, ruffled, morphologies after 30s, but showed an increasing proportion of polarized forms over the next 30 min.(ABSTRACT TRUNCATED AT 250 WORDS)